الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 138 |  |  |
| | | from | 138 | | |
Abstract
Resistance to carbapenems mostly develops due to production of carbapenemases which hydrolyze carbapenems as well as other lactams. Some of these carbapenemases include Imipenemase, Oxacillinase-48, Veron Integron Metallo lactamase, Klebsiella pneumoniae carbapenemases and New Delhi Metallo (NDM) lactamase which are encoded by blaIMP, blaOXA-48, blaVIM, blaKPC and blaNDM-1 genes. Carbapenemase-producing Enterobacteriaceae isolates have been increasingly identified worldwide.
Our study aimed to determine the occurrence of blaNDM-1 gene among clinical isolate of multidrug resistant gram-negative bacilli in Sohag University Hospital (a cross sectional laboratory-based study). Biological samples were collected from patients from different age groups and different types of infection admitted to the Outpatients Clinics of Sohag University Hospital.
Regarding the age and gender of the studied population, the mean age of the study population is 32.61±28.23 years and 62% of the samples were males. The sample was collected from three departments, medical department (which include internal medicine, GIT, chest & ICU) (26%), pediatric department (37%) and surgery department (37%). We found that the mean duration of hospitalization is 12.92±9.30 days with a median of 10 days.
We found that the diagnosis of the studied population differs as it was distributed as follow: 34% were diagnosed as SSI, 33% diagnosed as respiratory infection, 8% diagnosed as GIT infection, 3% diagnosed as PUO, 3% as dehydration, 2% for each of CHD, convulsion and stroke and 13 % for the remaining diagnosis.
Regarding the outcome of the studied population, we found that most of the study sample lived with good outcome with percentage of 82%.
We found that 25 % of the sample of the study population were sputum, 24% were pus, 23% were urine, 21% were blood and 7% for stool and pleural fluid.
Regarding the organism, we found that 33% of the study populations were Klebsiella pneumoniae, 24% were Escherichia coli, 16% were Pseudomonus aeruginosa, 14% were Enterobacter cloacae and 13% were other organisms.
As regard to the presence of NDM-1 gene, we found that more than half of the study populations were positive for NDM-1 gene with percentage of 53%.
Regarding the relation between organism and NDM-1 gene, our study showed that in most of cases the organism was Klebsiella pneumoniae (33%), 26 of them were positive NDM-1 gene (78.79%). This was followed by Escherichia coli (24%), 14 of them were positive NDM-1 gene (58.33%). Pseudomonus aeruginosa was found in (16%), 6 of them were positive NDM-1 gene (37.5%). Enterobacter cloacae were found in (14% of cases), 4 of them were positive NDM-1 gene (28.57%). There was a significant difference between groups regarding organism.