الفهرس 
Antibiotics from actinomycetesOnly 14 pages are availabe for public view
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Abstract
The subject of this thesis is based on the field of Microbiology. In this branch, we
concentrated production of Antimicrobial Agent by metagenomic techenique.
The thesis consists of seven chapters:
Chapter one: Introduction
It’s an introduction for the following chapters and contains some of the main
concepts and definitions which will use throughout our work.; also it illustrates the
metagenomics technique.
Chapter two: Review of literature
It’s an introduction for the following chapters and contains some of the main
concepts and definitions which will use throughout our work.; also it illustrates the
metagenomics technique.
The results of this chapter are published in Eco. Env. & Cons. Metagenomics technique as new
source for antimicrobial agent production.
Chapter three: Materials and methods
We present necessary and sufficient conditions of materials such as chemicals,
reagents, antibiotics, buffers, media, gels and stains, primers and vectors.
The results of this chapter are published in Eco. Env. & Cons. Metagenomics technique as new
source for antimicrobial agent production.
Chapter four: Results
Characterization of Antimicrobial agent was done for the best clone for
antimicrobial agent’s production to detect efficiency, optimum pH, thermal
stability, pH stability, the effect of different compounds on antimicrobial activity,
and residual activity of product after preservation at room temperature. The
amino acid sequence of RSMM C3 gene (1250 bp) was 72% identified with
Herbaspirillum sp. The ideal temperature level of the RSMM C3 antimicrobial
agent production was 36 ◦C. The antimicrobial agent RSMM C3′s stability was
stable at -20 ◦Celsius for up to two months without thawing. The antibacterial
agent RSMM C3 was stable at 4 ◦C for 14 days without loss in activity. The ideal
pH level of the RSMM C3 antimicrobial agent was 6. Remain activity was
gradually decreased at pH 5, 6, 6.5 and 7 (86.1, 96.9, 97.2 and 94.9%,
respectively). On the other hand, residual activity was (92 and 84%) at (pH 7.5
and 8) for 8 days. The tested antimicrobial RSMM C3 was stable against 1 mM of
different compounds (DMSO, Glycerol, NaCl, CaCl2, MgCl2, ZnCl2, FeSO4,
MnSO4 and CuSO4).
The results of this chapter are published in Biotechnology Reports. Evaluation of a new
antimicrobial agent production (RSMM C3) by using metagenomics approaches from Egyptian
marine biota.
Chapter five: Discussion