الفهرس 
General IntroductionOnly 14 pages are availabe for public view
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Abstract
Many vital compounds investigated throughout this work were determined in pharmaceutical preparations and biological fluids. These compounds include SOF, LED, SUL, AMP, PAR, MET and GLB.
The thesis consists of three chapters.
Chapter 1
This chapter is a general introduction about the pharmacological effect of these compounds, their structures, physical properties and solubility in different solvents.
Chapter 2:
Development and validation of simple HPTLC method for the determination of new hepatitis C subtype 4 antiviral agents in their tablet dosage form.
In this chapter, a new, simple, precise, accurate and selective HPTLC method has been developed and validated for the simultaneous determination of LED and SOF in tablet dosage form. chromatographic separation was carried out on Merck TLC aluminum sheets of silica gel 60 F254 using Ethyl acetate: Hexane: Methanol in the ratio of 8.00: 1.25: 0.75 (v/v/v) as mobile phase followed by densitometric measurement at 256 nm. The method was validated in terms of linearity, accuracy, precision, LOD, LOQ and specificity in accordance with International Conference on Harmonization (ICH) guidelines. The calibration curve was found to be linear between 60.00 to 1980.00 and 45.00 to 3600.00 ng/band for LED and SOF, respectively with significantly high value of regression coefficient (r²>0.9999) with linear and homoscedastic residuals. The limits of detection and quantitation were found to be 16.50 and 50.00 ng/band, respectively for LED and 13.00 and 39.50 ng/band, respectively for SOF. Comparative study was performed between the developed HPTLC method and the reported HPLC method. The quantitative results of both analytical methods did not show statistically significant difference, whereas the developed HPTLC method is both time and cost effective.