الفهرس 
HEMATOPOIETIC STEM CELL TRANSPLANTATION (HSCT)Only 14 pages are availabe for public view
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Abstract
Summary
Allogeneic hematopoietic stem-cell transplantation (allo- HSCT) is important and the only curative treatment for some malignant or benign hematopoietic diseases. Several factors are involved in determining the outcome of the graft including bone marrow microenvironment.
The current study was conducted to assess how the bone marrow microenvironment is being affected in patients with poor graft function post allogenic stem cell transplantation targeting the vascular endothelial cells using CD31+ antibody.
Forty patients were enrolled in our study with both benign and malignant hematological diseases. Patients were divided into two groups: group 1 includes 20 patients with poor graft function, group 2 includes 20 patients with good graft function
Patients were subjected to full history taking, full laboratory work-up then bone marrow aspirate was performed for histological testing and measuring CD31+ stem cells by multiparameter flow cytometry.
Analysis of demographic data and clinical characteristics revealed that sex mismatching, ABO incompatibility and major mismatch were significantly associated with PGF.
Analysis of hematological data revealed that PGF group showed significantly lower counts of WBCs, platelets, hemoglobin concentration and CD34+ cells compared to GGF group in addition to the presence of hypocellular marrow seen in the bone marrow examination post-transplant.
Analysis of percentage of stem cells with specific markers revealed that PGF group had statistically significant lower percentage of stem cells total count compared to GGF group. Concerning to the non-stem cells, PGF group had a significantly higher mean fluorescence intensity (MFI) of CD31 on non-stem cells compared to GGF group.