الفهرس | Only 14 pages are availabe for public view |
Abstract This study was conducted to investigate the prevalence of Salmonella species in processed meat as well as molecular typing and antibiogram of the recovered Salmonella isolates. A total of 200 processed meat samples (Sausage, burger, Kofta and luncheon) (50 of each) were randomly collected from different supermarkets and butchers in Suez Governorate, Egypt. The bacteriological examination was carried out, where the prevalence of Salmonella species was 18% (9/50), 10% (5/50), 6% (3/50) and 4% (2/50) for sausage, burger, kofta and luncheon, respectively. S. Typhimurium and S. Enteritidis were the most prevalent with a percentage of 5% and 2.5%, respectively in processed meat samples. In addition, the antimicrobial susceptibility testing was carried out. The PCR was used for detection of virulence genes in the isolated Salmonella serotypes, where all the tested serotypes were positive for invA and stn gene. While 84.2% harbored the hilA gene and 21% harbored the pefA gene. Moreover, the prevalence of strA gene was 47.4%. In conclusion, S. Typhimurium was the most prevalent Salmonella spp. in processed meat. The most prevalent virulence genes in Salmonella were invA and stn gene. While strA gene is the most prevalent antimicrobial resistance gene in Salmonella spp. isolated from processed meat products. |