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Abstract This work was conducted to investigate the hepato-nephroprotective effect of licorice extract and silymarin against cisplatin hepato- nephrotoxicity in rats. A total of 72 normal male albino rats body weight ranging from 150-200 gm. The animals were allowed to acclimatize for one week at the animal house. The rats were randomly assigned into 6 groups, 12 rats in each group, 12 rats were randomly selected as the normal control group and fed with commercial balanced diet. group (1), group (2): licorice extract oral treated group of 2gm/kg body weight daily for 6 weeks. group (3): silymarin oral treated group 100mg/kg daily for 6 weeks. group (4): served as hepatotoxic group treated with an injection of cisplatin intraperitoneally in a dose of 1.5 mg/kg body weight repeated twice a week for 3 weeks starting from the 4th week of experiment .group (5): received licorice extract oral treated group of 2gm/kg body weight and injected cisplatin intraperitoneally in a dose of 1.5 mg/kg body weight repeated twice a week for 3 weeks starting from the 4th week of experiment. group (6): received silymarin oral treated group 100mg/kg daily for 6 weeks and injected cisplatin intraperitoneally in a dose of 1.5 mg/kg body weight repeated twice a week for 3 weeks starting from the 4th week of experiment. Objective: The present study was carried out to investigate the effect of oral administration of extract of licorice and silymarin for 3 weeks on hepato - nephrotoxicity induced to rats by cisplatin (CP). These effects were explored by measuring relative weight of the liver and kidney , serum levels of liver enzymes; aspartate aminotransferase (AST), alanine aminotransferase (ALT); albumin, globulin, total protein, , cholesterol, bilirubin, glucose ,creatinine ,BUN,UA ,and antioxidant enzymes; reduced glutathione (GSH), , Malondialdehyde (MDA) and Immunological parameter as TNF- α, IL1- β, B2microglobulin, α-fetoprotein and CRP level, histopathological and Immunohistochemistry examination of liver and kidney were also performed. Abstract Results: The results showed that the oral administration of licorice extract and silymarin to intoxicated rats for 6 weeks significantly decreased the elevated serum levels of liver enzymes (AST, ALT), total bilirubin and increased serum total protein, albumin, when compared to the corresponding control positive groups. Levels of antioxidant enzymes glutathione reductase and superoxide dismutase were significantly increased as compared to the control positive groups.There was decrease in the elevated CRP, AFP, β2-MG, TNFα and IL-1β when compared to control positive groups. Histopathological examination of liver and kidney sections of rats pre-treated with licorice extract and silymarin showed alleviation of histological degenerative changes caused by cisplatin .the level of caspase-3 cleavage was reduced in pretreated groups compared with cisplatin Conclusion: The results suggest that, the licorice extract and silymarin induce potent hepato-nephoprotective and antioxidant effects in CP induced hepatonephrotoxicity in rats. This study recommends that intake of licorice and silymarin extracts as an herbal may be beneficial for patients who suffer from suffer from liver and kidney diseases caused by cisplatin usage and oxidative stress. |