الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Objectives: To (1) Determine the occurrence of Listeria species in poultry farms, supermarkets, poultry products (chicken meat and eggs) and poultry products handlers in the examined area. (2) Assess the presence of some virulence genes among the identified L. monocytogenes strains. (4) Determine the antibiotic susceptibility profile of the recovered Listeria species. Design: Descriptive study. Sampling: The study comprised 350 samples collected from different sources including cloacal swabs from different poultry species (n =200), poultry carcasses (n = 25), poultry products (25 samples each of chicken luncheon and frozen chicken breast fillets), chicken eggs (n = 50) and stool specimens from the contact workers (n = 25). The cloacal swabs were collated from different poultry species including broilers (n = 40), layers (n = 40), ducks (n = 40), pigeons (n = 40), and turkeys (n= 40). Procedures: All samples were examined using conventional bacteriological and molecular techniques. Results: The obtained results showed that, Listeria spp., was identified in 17.14% (60/350) from the examined samples. Of which,15.5% (31/200) were recovered from the cloacal swabs; while 22.5%, 22.5%, 12.5%, 10%, and 10% were isolated from broilers, layers, ducks, pigeons, and turkeys, respectively. The most predominant Listeria spp. identified in the examined cloacal swabs were L. ivanovii (n= 10, 32.3%), L. innocua (n= 9, 29%) and L. monocytogenes (n=6, 19.4%). L. monocytogenes was identified in 19.4% (6/31) from the examined cloacal swabs. the detection rate of Listeria spp. among the examined eggs samples was 24% (12/50), in which 36% (9/25) were recovered from eggshells, while 12% (3/25) were identified in eggs contents. Two samples out of 25 (8%) were positive for Listeria spp. in the examined stool specimens of poultry handlers. All isolated L. monocytogenes expressed resistance to most of the tested antibiotics where isolates originating from chicken luncheon, eggshells and layers’ cloacal swabs exhibited multiple antibiotic resistances. L. monocytogenes showed high resistance to nalidixic acid (100%) followed by neomycin (85.1%), streptomycin (80%), cefotaxime (65%) and penicillin g (58.3%). followed by vancomycin and clindamycin (78.9%), Sulphamethoxazol and chloramphenicol (68.4%). Key Words (not more than 10)Listeria, L. monocytogenes, Poultry, Zoonoses, Molecular characterization, Antibiogram Profiles, iap, hylA, actA. ”