الفهرس 
Phytochemical and biological studies of acacia auriculiformis (A. Cunn.) ex Benth. and Acacia pennata (Linn.) Willd.,family fabaceae
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Abstract
Acacia auriculiformis (A. Cunn.) ex Benth. and Acacia pennata (Linn.) Willd. belonging to the family Fabaceae, are plants of diverse medicinal importance, and have been employed for various ailments in traditional medicine. Reviewing the available literature on both plants, nothing was traced concerning the phenolic phytoconstituents of A. auriculiformisleaves. However, some flavonoids were reported from A. pennata leaves. The total phenolic and flavonoid contents of the total and defatted leaves extracts of both plants were determined (Folin-Ciocalteauand Aluminum Chloride colourimetric assays). The highest total phenolic and flavonoid contents were exhibited by the defatted A. auriculiformisleaves extract (256.6 mg GAE/g and 125 RE/g, respectively), followed by the defatted A. pennata leaves extract (194.5 mg GAE/g and 118.75 mg RE/g, respectively). Whereas, the total phenolic and flavonoid contents of the 70% methanol extract of A. auriculiformis (130.6 GAE/ g and 54.75 mg RE/g, respectively) and A. pennata (107.75 mg GAE/ g and 45.6 mg RE/ g) were found to be lower than their respective defatted extracts. In this study, A. auriculiformis was subjected to different chromatographic techniques to investigate its phenolic constituents. Twelve known phenolic compounds were isolated, with additional six flavonoidswhich were tentatively identified from its defatted leaves extractfor the first time using LC-ESI-MS technique.The phenolic profiling of the defatted A. pennata leaves extract revealed the tentative characterization of nine flavonoids by LC-ESI-MS analysis, seven of which were detected for the first time.HPLC-UV chromatographic quantitation for the isolated flavonoids: quercetin 3-O-glucoside, eriodicotyl 7-O-glucopyranoside, genisteinandbiochanin A were determined as 0.215, 0.149, 0.412 and 0.371 mg/g A. auriculiformis leaves powder, respectively