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العنوان
Impact of using nano-particles during date palm tissue culture stages /
الناشر
Amany Abdou Kinawy ,
المؤلف
Amany Abdou Kinawy
هيئة الاعداد
باحث / Amany Abdou Kinawy
مشرف / Samy ElKosary
مشرف / Abdou Mohammad Abdallatif
مشرف / Mona Mohammad Hassan
تاريخ النشر
2021
عدد الصفحات
111 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البساتين
تاريخ الإجازة
9/5/2020
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Pomology
الفهرس
Only 14 pages are availabe for public view

from 136

from 136

Abstract

This study aimed of produce date palm plants from the immature inflorescences using two cultivars of Sewi and Medjool through tissue culture technique during the period 2017-2020.The response of explants was studied effecting by different nanoparticles Ag, ZnO and MgO and also, the effect of chitosan and tungsten as micrometric substances. The results showed Ag NPs had effective to completely eliminate plant microbial contaminates at sterilizing by 250ogL⁻¹ for 10 min without affecting survival rate compared HgCl₂. Furthermore, 125ogL⁻¹ is the best for the embryo formation, multiple and germination while 500ogL⁻¹ recorded highest rate of callus formation and growth. The results showed 125ogL⁻¹ of chitosan and tungsten was the best for embryo formation. 500ogL⁻¹ achieved the highest rate of callus formation and growth and increasing embryo multiple and germination rate. The plantlets response rates when adding both ZnO NPs and MgONPs were more effective than added ZnSO₄ and MgSO₄ in culture media. 0.1mgL⁻¹ ZNONPs recorded the highest rate of embryo multiple, leaves no. and number of root, length and thickness, this explains the decreasing IAA and chl a,b with increasing ZnONPs concentration. While 6mgL⁻¹ of MgONPs is the highest in terms of embryo multiple, germination, number of leaves, root and length