الفهرس 
Title : DNA Analysis Of Challenging Forensic Samples Models Toward Fully Integrated Short Tandem Repeats
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Abstract
Most of the forensic genetic analytical methods used today employ short tandem repeats (STRs) to provide discriminatory power. The data showed that different factors may affect STR profiles in challenging samples including DNA quantity, DNA quality, PCR inhibitors and storage time. In the present study, dried bloodstained samples on two types of stained fabrics (black cotton and denim), contain challenging dyes, have been used to compare the efficiency of two different DNA-extraction methods (automated magnetic beads based method (EZ1) , and the organic method), with and without using carrier RNA molecules, to assess the quality and quantity of the extracted DNA, and their capabilities for producing reportable STR-profiles, at two storage time.Carrier RNA was added following cell lysis, that caused a dramatically increase in DNA recoveries on the EZ1 in contrast to organic method. EZ1 was found to be preferred than organic especially when a time passed over. Organic method preferred when samples are available in small quantities. In addition, using carrier RNA during the organic method steps showed no improvement in STR profiling. EZ1 with carrier RNA was preferred for bloodstain samples on fabrics with textile dyes (black dye or denim indigo), especially at long storage. Denim was more problematic than Cotton due to presence of challenging inhibitors (indigo dye) which lead us to use EZ1 method other than using organic method with inhibitory components like phenol. A better understanding of these inhibitory samples could lead to the development of more sensitive, more robust analytical protocols