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العنوان
Genetical studies of some cultivated Arab date palm varieties using different pollinators /
الناشر
Amira Hassan Mohamed Hassan ,
المؤلف
Amira Hassan Mohamed Hassan
هيئة الاعداد
باحث / Amira Hassan Mohamed Hassan
مشرف / Mona Hashem A. Hussein
مشرف / Salah Eldin Sayed Elassal
مشرف / Sayed Gomaa Ibrahim
تاريخ النشر
2021
عدد الصفحات
127 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
26/12/2021
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Agricultural Sciences
الفهرس
Only 14 pages are availabe for public view

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from 161

Abstract

Date palm (Phoenix dactylifera L.) is an important and oldest fruit tree. Determination of genetic relationships between date palm cultivars is very useful for characterization of date palm germplasm, breeding programs, conservation purposes and genetic improvement. In this work the PCR-based markers (ISSR and SRAP) have been used as tools to determine the relationships between date palm cultivars that are difficult to distinguish morphologically. Results revealed that the total polymorphism detected by the ISSR assay (59.02%) with a total polymorphic bands of (36) was higher than that observed for SRAP (51.85%) with a total polymorphic bands of (56).On the other hand, we have attempted to determine the sex-specific DNA markers for some date palm cultivars and selected progeny using molecular techniques (RAPD and ISSR) to detect the sex in date palm seedlings at early stage. Using both RAPD and ISSR analyses gave one positive specific marker for female and eight for male in RAPD analysis in addition to five positive specific markers for female and three for male in ISSR analysis and the level of polymorphism between cultivars was 77.01% and 80.45% as revealed by RAPD or ISSR, respectively. Moreover, the gene expression investigations aimed to evaluate the gene expression for the three genes, Phosphofructokinase, pectin (pectate) lyase and Xyloglucan (Xyloglucosyl transferase) for fruit quality of date palms (Barhee and Majdool) in Khalal stage