الفهرس 
Title : Probiotics and bacteriological aspect of bovine milk
AbstractOnly 14 pages are availabe for public view
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Abstract
Bovine mastitis is a costly disease in dairy farms globally. The control of such disease is based on prevention by applying strict hygienic measures during milking. Other approaches include vaccination and application of antibiotics. Regardless of these procedures, mastitis is not entirely under control, thus increasing the need for alternative tactics. This study was conducted to isolate and identify lactic acid bacteria (LAB) from fresh cow{u2019}s milk which possess antibacterial activity that could be used for mastitis control. A total of 146 isolates were recognized as lactic acid bacteria (LAB) from105 tanks milk samples after being cultured anaerobically on selective media MRS agar plates for 48 hours at 37oC and identified by general bacteriological investigation. Afterwards, 24 isolates were identified to belong to genus Lactobacillus using Polymerase chain reaction (PCR). For species level recognition, MALDI-TOF MS was used and resulted in the identification of: (5) L. fermentum, (3)L. brevis,(4) L. plantarum,(2) L. paracasei,(3) L. rhamnosus,(2) L. pentosus,(3) L. casei,(1) L. raffinolactis and(1) L. mesenteroides. The antimicrobial activity of the isolated strains against one of the major mastitis pathogens, S. aureus,were detected by agar well diffusion assay and modified double layer method. L. casei, L. fermentum and L. plantarum possesses the most inhibiting effect besides they have no hemolytic neither gelatin liquefaction activity when their safety profiles were evaluated. The result of the antibiotic susceptibility test revealed that these isolates were resistant to vancomycin (VA), neomycin (N) and gentamycin (CN). on the other hands, they were highly sensitive to amoxicillin clavulanic acid (AMC), levamisole (LEV), tetracycline (TE) and penicillin (P).The study suggested that some of the L. casei, L. fermentum, L. rhamnosusand L. plantarum strains are perfect candidates to be used as probiotics in preventing and controlling bovine mastitis as they were proven to be safe and have antimicrobial activity against S. aureus