الفهرس | Only 14 pages are availabe for public view |
Abstract Breast cancer is a heterogeneous disease with a wide difference in molecular and morphological subtypes that are variable in behaviour and response to therapy. Silver nanoparticles have a special role in modern anticancer therapy, as they are widely used in detection and diagnosis of malignant tumors as well as in drug delivery systems. Green biosynthesis of silver nanoparticles using microorganisms is considered a better strategy than traditional physical and chemical synthesis methods, which are expensive, energy-consuming, and hazardous to the environment. The present study is divided into two main parts: the first part was an experimental study, in which the toxicity of green biosynthesized AgNPs by bacteria (Streptomyces malachitus) has been investigated in vivo in pregnant female rats and their fetuses, and in vitro using two different types of breast cancer cell line (MDA-MB 231 & MCF7). The second part was a molecular study, in which the common phenotypic features of nucleoli in cohorts of breast cancer (BC) patients have been correlated with the underlying molecular pathways (genotype), then the prognostic importance of some genes in these molecular pathways have been assessed as possible vital biomarkers. from the experimental part, it could be concluded that chitosan coating reduces the toxicity of green biosynthesized AgNPs, making it possible to use them as a drug delivery tool to target fetuses in the womb. Also, AgNPs produced from Streptomyces malachitus are promising agents in development of reliable and effective anticancer drug in treatment of breast cancer. We conclude from the molecular part that DKC1 expression is strongly correlated with nucleolar prominence and its overexpression in BC is associated with unfavourable clinicopathological characteristics and poor outcome. |