الفهرس 
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Abstract
The current investigation was performed in two consecutive seasons, 2017 and 2018 on forty five vines which are 6 years old on Superior seedless grapevines grown in a private vineyard located at Talla village, Minia Governorate, where soil texture is clay and well drained since water table depth is not less than two meters deep. Vines are planted at 1.5 x 3.0 meters apart (933vines/ fed.) pruned during the last week of December in both seasons. The cane pruning method with Gable supporting system was followed, leaving 96 buds/ vine on the basis of eight fruiting canes with 10 buds with eight renewal spurs x two buds. Surface irrigation method was followed using Nile water. Common horticultural practices were carried out as usual.
This study aimed to examine the influence of single or in combination with three sprays of amino acids namely (glutamic acid, glycine and aspartic acid), vitamins B complex and boron on vegetative growth, vine nutritional status and fruiting of Superior seedless grapevines.
This study included the following:
1- Control (sprayed with tap water).
2- Spraying with glutamic acid at 250 ppm.
3- Spraying with glycine at 250 ppm.
4- Spraying with aspartic acid at 250 ppm.
5- Spraying with vitamins B (B1 + B2 + B6 + B12) at 100 ppm.
6- Spraying with boric acid at 0.05%.
7- Spraying with glutamic acid + vitamins B
8- Spraying with glycine + vitamins B
9- Spraying with aspartic acid + vitamins B.
10- Spraying with glutamic acid + Boric acid.
11- Spraying with glycine + Boric acid
12- Spraying with aspartic acid + Boric acid.
13- Spraying with glutamic acid + vitamins B + Boric acid
14- Spraying with glycine + vitamins B + Boric acid
15- Spraying with aspartic acid + vitamins B + Boric acid.
Each treatment consisted of three replicates, one vine / each. Three amino acids (glutamic acid, glycine and aspartic acid) vitamins B complex (B1 + B2 + B6 + B12) and boric acid 0.05 %) were sprayed thrice: once at growth beginning (1st week of March), and once after berry set (1st week of April) and at month later (1st week of May). Triton B as a wetting agent was added to all the spraying solutions at 0.05 %. Spraying was done till run off.
The experiment was set up as Complete Randomized Block Design (CRBD) included fifteen treatments and each treatment was replicated three times, one vines per each.
The next measurements at the two experimental seasons, were estimated;
1- Some vegetative growth aspects e.i: length of shoot (cm), leaves No./ shoot, area of leaf (cm)2, wood ripening coefficient, weight of pruning wood (kg.) and thickness of can (cm).
2- Chemical components of leaf included: chlorophyll A (mg/ 1 g F.W.), chlorophyll B (mg/ 1 g F.W.), total chlorophylls in vines (mg/ 1 g F.W.), carotenoids (mg/ 1 g F.W.), leaf N %, P %, K %, Mg %, Zn ppm, Fe ppm and Mn ppm.
3- Yield / vine as a weight (kg.) and number of cluster per vine as well as weight (g.), length and shoulder (cm.) of cluster.
4- Shot berries % and physical traits of the berries i.e: weight of berry (g.), berry width (cm) and berry length (cm).
5- Some chemical properties of the berries included: % of total soluble solids, % reducing sugars and % total acidity.
1-Some vegetative growth aspects:
Spraying one of the three amino acids, glutamic acid, glycine, and aspartic acid, each at 250 ppm, or vitamins B complex at 100 ppm, or boric acid at 0.05% improved the six vegetative growth characters, namely length of shoot, leaves No. /shoot, area of leaf, wood ripening coefficient, weight of pruning wood, and thickness of cane. Promotion was related to the increase effected by the combination of glutamic acid, glycine, and aspartic acid in descending order with vitamins B and boric acid.
The maximum values were registered on the vines that gave 250 ppm glutamic acid, 100 ppm vitamins B complex and 0.05 % of boric acid.
2- Chemical components of leaf:
Some leaf chemical components namely chlorophyll A, chlorophyll B, total chlorophylls in vines, carotenoids (mg/ g F.W.), N, P, K, Mg, (as %) Zn, Fe, Mn (as ppm) were greatly improved due to spraying one of the amino acids (glutamic acid, glycine and aspartic acid) in descending order. Each at 250 ppm vitamins B at 100 ppm and boric acid at 0.05 % compared with the control.
Using the previous chemicals separately has less effect than using combination of them. The maximum values of leaf contents of the plant pigments and nutrients were obtained on the vines that sprayed thrice with combined of glutamic acid at 250 ppm + vitamins at B 100 ppm + boric acid at 0.05%.
3- Yield/ Vine and cluster aspects:
The yield, weight and dimensions of clusters were considerably increased due to using some amino acids alone or with vitamins B complex or boric acid compared to untreated one (control). The best used of amino acids were glutamic acid followed by glycine then aspartic acid in descending order. Applying amino acid was very effective on Superior seedless than using each vitamins B and boric acid. The best results related the yield and cluster weight and dimensions were observed due to spray glutamic acid at 250 ppm plus vitamins B at 100 ppm plus boric acid at 0.05% three times. The lowest values were recorded on check treatment.
4-Percentage of shot berries:
There was a remarkable decreasing in the shot berries percentage, due to one of the amino acids alone or with vitamins B alone or with boric acid rather than control. The reduction in the shot berries % was considerably related with using amino acids namely glutamic acid, glycine and aspartic acid in descending order. Spraying amino acids was very effective in Superior seedless than using either vitamins B or using boric acid in this respect. Lowest values were obtained on the vines that sprayed with glutamic acid, vitamins B and boric acid together three times.
5- Some physical and chemical characteristics of the berries:
All physical and chemical characteristics of the berries were positively affected by using one of the three amino acids (glutamic acid, glycine and aspartic acid) alone or with vitamins B complex alone or with boric acid rather than control. This promotion appeared in expression of excessing berry weight and dimensions, % of T.S.S., % of reducing sugars and T.S.S./ acid ratio and in lessening total acidity. Spraying one of the amino acids was prevailing than vitamins B and boric acid, using one of the amino acids, vitamins B and boric acid was favorable in promoting berry quality the than using each alone. The best quality of berries were recorded due to spray the vines three times with combined of glutamic acid + vitamins B + boric acid.
CONCLUSION
Under the conditions of this experiment, it is advised to spray Superior seedless grapevines three times at growth beginning, just before berry set and one month later with combination of glutamic acid at 250 ppm plus vitamins B complex (B1 + B2 + B6 + B12) at 100 ppm and 0.05 % boric acid to increase yield and improve berry quality.