الفهرس 
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Abstract
In the present study, a total of 400 samples; collected from sheep and goat; were processed for isolation and identification of Mycoplasma species, microbiologically and by molecular techniques. Out of the examined samples, only 43 Mycoplasma isolates (10.75%) were identified as M. ovipneumoniae, M. arginini and untyped Mycoplasma species. M. arginini showed the highest isolation rate from both sheep and goat with incidences of 58.3% and 58.1%, respectively. While, the incidence of M. ovipneumoniae was 11%, and 30% for the untyped Mycoplasma species. Confirmation of the isolates was done by PCR then by sequencing of the PCR products. The sequence results of 5 M. arginini sheep isolates, 4 M. arginini goat isolates, 4 M. ovipneumoniae sheep isolates, one M. ovipneumoniae goat isolate were all submitted to the Genbank taking the accession numbers; MK291433, MK291434, MK291435, MK291436, MK291437, MK640677, MK640679, MK643127, MK774823, MK300052.1, MK 300042.1, MK 361039.1, MK 361029.1 and MK300051.1, respectively, in addition to one untyped Mycoplasma species with the accession number MK910041.The phenotypic virulence traits of sheep and goat isolates including hemolytic activity, biofilm formation, hydrogen sulfide production and catalase enzyme activity were tested. Mycoplasma species isolates showed weak adherence ability to the polystyrene multiwall plates. Phenotypically, the hemolytic activity was recorded in 18 sheep isolates and 13 goat isolates. The antibiotic susceptibility testing by MIC showed sensitivity of both sheep and goat isolates to tulathromycin, tylosin, streptomycin and oxytetracycline. All the isolates were resistant to lincomycin. H2S was detected in eight sheep isolates and 5 goat isolates. Genotypic characterization of the tested positive isolates showed negative results for fluoroquinolone and macrolide resistance genes (gyrB, parC, parE, 23S rRNA D2, 23S rRNA D5, rplD, and rplV). The present study highlights the role of Mycoplasma species affecting sheep, goat. To our knowledge, it is the first record for biofilm production by Mycoplasma isolates, also, the first record of studying the hemolytic activity of Mycoplasma species of veterinary origin.