الفهرس | Only 14 pages are availabe for public view |
Abstract This work deals with the taxonomic, molecular, and phylogenetic studies on the Egyptian fauna of family Hydraenidae and covers the following points: 1- Taxonomic studies: A comprehensive taxonomic study was carried out on family Hydraenidae in Egypt. This study was based on the species collected through visits to 15 governorates during the period from 2018-2020, as well as the specimens preserved in the main Egyptian Reference Collections and those recorded in literature. According to this study: ▪ Our fauna comprises 17 species within three genera; Ochthebius Leach, Limnebius Leach, and Hydraena Kugelann which are belonging to two subfamilies Hydraeninae and Ochthebiinae. ▪ Two newly recorded species (Ochthebius micans BalfourBrowne and Hydraena arabica Balfour-Browne) are identified. ▪ Six species were doubted to be included in the Egyptian fauna (Hydraena quadricollis Wollaston, Ochthebius difficilis Mulsant, O. rugulosus Wollaston, O. minimus Fabricius, Limnebius frontinalis Balfour-Browne, and L. 163 nitidus Marsham) according to the world catalogue of insects by Hansen (1998) and the catalogue of Palearctic coleoptera by Jäch (2004). ▪ Keys and diagnoses are given to all taxa. Type localities, synonyms, specimens examined, local & geographical distributions, distributional maps and photos for each species are provided. 2- Molecular and Phylogenetic Studies: A. Hydraenidae The PCR product of CO1 gene was approximately 680 bp. The PCR products of Hydraena arabica, Ochthebius lividipennis, O. merdionalis, and O. thermalis have been sequenced from both forward and reverse directions. The evolutionary relationship among the four hydraenid species was demonstrated by a phylogenetic tree based on the partial nucleotide sequences of CO1 gene, and this phylogenetic tree revealed that these species are clustered into two major groups. B. Wolbachia Our Wolbachia phylogeny clarifies the clustering between non taxonomically related beetles, which indicates the lack of coleopteran host specificity to 164 Wolbachia and proves the low degree of codivergence between Wolbachia and beetles’ ancestors. Besides, there is an obvious correlation between Wolbachia supergroups B and F within the beetles’ host, this tree also indicates that supergroup F is the common ancestor of the other Wolbachia supergroups |