الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 109 |  |  |
| | | from | 109 | | |
Abstract
Salivary gland tumors include a heterogeneous group of benign and malignant neoplasms with significant variability in their microscopic appearance as well as biological behavior, and their clinical presentation can vary according to the lesions. Limited investigations were accomplished on malignant salivary gland tumors (MSGTs) to study their invasive potentials and proliferative activity. Among these investigations, AgNOR staining and DNA analysis using FCM. DNA analysis by flow cytometry provides data regarding DNA content, and also SPF while AgNOR staining are valuable as marker of cellular proliferation.
In our current study, we evaluated AgNORs count in malignant salivary gland tumors. Moreover, we evaluated the DNA ploidy using FCM and correlated the results of both techniques with clinical parameters.
The current study included 47 malignant formalin fixed paraffin embedded (FFPE) tissue blocks of SGTs which had been encountered in Oncological Pathology Departments, SECI, Assiut University during the period from 2014-2018. They were diagnosed as twenty-six cases of MEC, fifteen cases of ACC, four cases of MECA and 2 cases of AciCC. Moreover, the collected samples were serially cut into approximately 5 μm thick sections and stained routinely with H&E stain for re-evaluation of the diagnosis.
Silver nitrate was used for AgNOR staining. For DNA analysis by FCM, single cell suspension from FFPE tumors was prepared according to a modified method used by Leers et al(1999) using DNA cycle test kit (Cycle Test ™ Plus DNA Reagent Kit (BD Bioscience)).
This study showed that from the 47 cases studied, 28 (59.6 %) were DNA diploid, and 19 (40.4%) were DNA aneuploid. A significant association was present between DNA ploidy and each of tumor site, size, and type. SPF was significantly associated with LVI. On the other hand, AgNORs count was statistically significant with tumor type. No significant association was noted between DNA ploidy, TSPF and each of Patient’s age, PNI and LNM.
This study showed that a moderate positive correlation was present between AgNORs count and TSPF. A moderate negative correlation was present between DNA ploidy and TSPF. No significant correlation was found between AgNORs count and DNA ploidy.
There was a significant association between LNM, tumor site, DNA ploidy, AgNORs count, poor overall survival (OS) and disease free survival (DFS).