الفهرس 
Antimicrobial review of different root canal sealers using dentin infection modelsOnly 14 pages are availabe for public view
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Abstract
This In-Vitro study was conducted to evaluate the penetration ability of two different types
of sealers; a calcium silicate based root canal sealer (Endosequence BC Sealer) and an epoxy
based resin sealer (AH Plus sealer). Furthermore; to assess the antimicrobial ability of these two
sealers over a thirty day period in eradicating the bacterial contamination inside the dentinal
tubules of the root canal, using an in vitro model contaminated with Enterococcus faecalis.
Seventy five freshly extracted single rooted teeth were selected for this study.
Standardized root segments of a length of seven mm were prepared. 56 specimens underwent
antimicrobial testing while 18 specimens underwent penetration ability testing.
For antimicrobial testing, following smear layer removal and sterilization the 56
specimens were infected with Enterococcus faecalis (ATCC 29212) suspension using
centrifugation and incubation for three weeks. The specimens were then randomly allocated
into three groups with 18 specimens in each group according to the sealer to be tested. The
specimens were obturated using cold lateral obturation and embedded one mm into a preprepared Brain Heart infusion agar plate. Each group contained three subgroups with six
specimens in each subgroup according to the testing period performed (one day, seven days and
thirty days). After each testing period, dentin powder was collected from within the canal lumen
using sequential removal via the use of peeso reamers sizes three, four, five and six.
Proportions of remaining bacteria inside the dentinal tubules at each ISO Size after exposure to
the root canal sealers were assessed by Colony forming units (CFU) count on blood agar plates.
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For penetration ability testing, following smear layer removal the specimens were
randomly divided into three main groups with 6 specimens per group according to the sealer
to be tested. The specimens were obturated using cold lateral obturation and incubated for two
weeks at 37˚C in 100% relative humidity. Following the incubation period the specimens
were then transversely sectioned and prepared to be observed under the scanning electron
microscope. Minimum and maximum sealer penetration depths were measured and tabulated.
Results were tabulated and statistically analyzed using suitable statistical tests.