الفهرس 
Hot springsOnly 14 pages are availabe for public view
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Abstract
Isolation of microorganisms from water and sediments samples obtained from the hot spring located in Ras-Sedr using different culture media was carried out and ATTC medium showed the heights count. This step was followed by evaluation of the capacity of the obtained isolates for hydrolytic enzymes production. Isolates showed the ability for growth at different temperature and pH values with differentiation in the optimum conditions for growth. According to BLAST analysis; molecular identification revealed that bacterial isolates has a high degree of sequence similarity with the genera Anoxybacillus, Geobacillus and Bacillus and actinobacterial isolates has a high degree of sequence similarity with genus Thermoactinomyces. Powerful isolates were selected as source of enzymes, and then the assessment of the impact of pH & temperature on activity and stability of these enzymes were carried out. Metagenomic DNA was extracted from both water and sediments of the hot spring of Ras-Sedr. Lipolytically active clone was subjected to plasmid isolation. LipC12 gene sequence was identified using NCBI BLAST server and purified using Ni2+ column. Electrophoresis of protein was done with SDS-PAGE and protein size confirmed to be 30 kDa. The effect of pH and temperature on both activity and stability of recombinant LipC12 lipase revealed that recombinant lipase maximum stability was at the pH range from 7 to 10 with the highest activity at pH 9 and the maximum activity was obtained at 55°C.