الفهرس 
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Abstract
Clostridium perfringens in meat have a public health importance as food poisoning organism. C.perfringens were isolated from 120 examined meat samples from cattle, camel and sheep (20 for fore quarter, 20 for hind quarter) by ratio 25 % and 35%, 30% and 45%, 25% and 40% from fore and hind quarter of cattle, camel and sheep meat samples respectively. The identification and characterization of isolated C.perfringens depend on culturing method, dermonecrotic reaction in guinae pigs and application of multiplex PCR for toxin typing. Molecular typing of the major toxin genes of C.perfringens by multiplex PCR revealed that positive gene amplification at 324 bp for C.perfringens type A. Also enterotoxin virulent gene was detected at 485 bp. This study concluded that multiplex PCR is rapid, accurate and sensitive method for typing of C.perfringens toxins and detection of enterotoxin gene.