الفهرس 
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Abstract
Salmonella is still the major threat to the poultry industry and humans
especially that of zoonotic importance. In the present study, a total of 300
samples (liver, intestine, yolk sac and spleen) collected from 100 broiler
chickens were examined bacteriologically for the presence of Salmonella.
The isolated salmonellae were then screened for virulence encoding
genes using multiplex PCR and the antimicrobial susceptibility to
antibiotics using disc diffusion method. Results showed that Salmonella
was recovered from 5.33% of the examined samples. Serotyping of
Sixteen Salmonella serovars recovered [Salmonella Sinchem (n=3)
Salmonella Typhimurium (n=2), Salmonella Gallinarum (n=2),
Salmonella Enteritidis (n=2), Salmonella enterica subsp. Salamae (n=1),
Salmonella Virchow (n=1), Salmonella Kentucky (n=2), Salmonella
Heidelberg (n=1), Salmonella Farsta (n=1) and Salmonella Hydra
(n=1)]. Results also showed that all the tested salmonellae (100%) were
found harbor the virulence encoding gene specific amplicon of pagC,
msgA, spiA, invA, prgH, orgA, sipB, tolC, iroN, lpfC, pefA, sitC, sifA, and
sopB. While, only 30% and 70% of the examined salmonellae were
harbor cdtB and spvB, respectively. The antimicrobial susceptibility
testing of the isolates revealed that most of the isolated Salmonella
serovars were expressed antibiotic sensitivity to doxycycline,
chloramphenicol, ampicillin, gentamicin, Trimethoprim /
sulphamethoxazole and amoxicillin. In conclusion, the results of the
current study demonstrated that Salmonella isolated from broilers chicken
were found to harbor many virulence encoding genes.