الفهرس | Only 14 pages are availabe for public view |
Abstract Reports illustrating the diversity of trichostrongyles infecting small ruminants from Egypt are confusing and lack the definitive methodologies for species characterization. In the present study, molecular and Egg-lectin binding approaches were employed for accurate identification of the most economically important trichostrongyles in 17 mixed herds of sheep and goats from 6 localities in Dakahlia governorate, Egypt. For species identification, eggs from 75 (63 sheep and 12 goats) high egg count samples and pooled eggs (n=20 pools, 16 sheep and 4 goats) from moderate or low egg count samples were examined. Molecular examination was conducted amplifying the ITS2 region of the rDNA for 6 different trichostrongyles in individual PCR reactions, whereas 4 fluorescently-labeled specific lectins were used for Egg bindings: peanut agglutinin (PNA) for Haemonchus contortus, Aleuria aurantia agglutinin (AAL) for Trichostrongylus species, Lens culinaris agglutinin (LCA) for Teladorsagia circumcnicta and Lotus tetragonolobus lectin (LTL) for Cooperia species. Findings of the earlier reports from Egypt were tabulated and reviewed. Fourteen (82.3%) herds were found infected, of which trichostrongyle eggs were detected in feces of 26.5% (173/653) of sheep and 10.2% (21/205) of goats. Four trichostrongyles were detected: H. contortus, T. circumcincta, T. axei and T. colubriformis. The present study provides accurate identification for the most economically important trichostrongyles infecting sheep and goat herds in Dakahlia governorate, Egypt, and emphasizes the basic role of H. contortus in small ruminants` trichostrongylosis in this area; the parasite was found in all the infected herds, and their eggs were dominant over eggs of the other detected trichostrongyles. |