الفهرس 
cover englishOnly 14 pages are availabe for public view
 |  | from | 114 |  |  |
| | | from | 114 | | |
Abstract
Bovine respiratory disease is a major cause of economic losses in dairy beef and feedlot cattle due to increase mortality rate, intensive cost of treatment and prevention in addition to reduction of carcass value . In the present work it was planned to improve the control of these infections through acceleration of the field identification of some of the important pathogens causing these infections. This work was planned for development of simple rapid field test. The developed test was designed for identification of two members of the Pasteurellaceae family, Mannheimia haemolytica (formerly Pasteurella haemolytica biotype A) and Pasteurella multocida, these two species are frequently associated with BRD.In the present work a fully identified pure culture of P. multocida and M. hemolytica was obtained and a pathogen specific antigens was prepared. P. multocida and M. hemolytica specific polyclonal antibodies were prepared raised in rabbits and guinea pig .The pathogen specific IgG was purified by caprylic acid method and the titer of this pathogen specific IgG was determined using ELISA. The pathogens specific rabbit polyclonal IgG antibodies were conjugated with colloidal gold nanoparticles and used for preparation of lateral flow kits for detection of P. multocida and M. hemolytica. The sensitivity, specificity and accuracy of the developed kits were determined using bacteriological examination as astandard gold test It was 88.2%, 92.4% and 91% respectively For P. multocida and for M. hemolytica 80.6%, 94.2% and 90% respectively.