الفهرس 
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Abstract
The aerosol generated from E-liquid of electronic cigarettes led to occurrence of adverse side impacts and oxidative damages on major salivary glands which played an essential role in preserving oral cavity and dental health. Vitamin C was considered as a potent antioxidant that was able to remediate oxidative stress by performing a prophylactic role in preserving against generation of free radicals caused by the electronic cigarettes. The objective of this study is to evaluate the impact of electronic cigarette liquid on rats’ major salivary glands with/without administration of ascorbic acid. Methods Twenty four adult male albino rats were randomly divided equally into three groups. group I, animals were injected intra-peritoneally daily with normal saline. In group II, animals were injected intra-peritoneally daily with E- liquid containing 0.5 mg of nicotine/kg for 6 consecutive days. The administration continued day after day till the end of the month. In group III, animals were injected intra-peritoneally daily with E-liquid containing 0.5 mg of nicotine/kg and one hour after E-liquid injection, they received vitamin C 300 mg/ kg by gastric gavage for 6 consecutive days. Then animals received the same dose of E-liquid and vitamin C day after day till the end of the month then, euthanized and sacrificed of the animals were done. The glands were assessed histologically, ultrastructurelly, and immunohistochemically for Ki-67 for evaluating the acinar cells proliferation and alpha smooth muscle actin for expressing myoepithelial cells. Results The histological sections in group II revealed severe histological changes in parotid and submandibular glands respectively in comparison to sublingual gland. While in group III, sections revealed ameliorated impact of vitamin C on the glands against the damage induced by electronic cigarettes. The SPSS Statistics revealed an overall significant difference between different groups (p <0.05). The immunohistochemical sections of αSMA stain in the 3 glands revealed weak, intense and moderate immunocytoplasmic reactivity in groups I, II, and III respectively. While immunohistochemical sections of Ki-67 stain in 3 glands revealed strong, weak, and moderate nuclear reaction in acini and ducts in groups I, II, and III respectively. Conclusion The results presented by this study give supplementary data to argue the hazardous effects of ECs on major salivary glands while the consumption of vitamin C ameliorated these glands from oxidative damage induced by ECs.