الفهرس | Only 14 pages are availabe for public view |
Abstract Liver cancer is the sixth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide. Nowadays, natural products represents a good source of anticancer drugs. In this study, six sediment samples were collected from lsmailiyah canal in Cairo, and one sediment was collected from Ras Mohamed, Sharks Bay, South of Sini, Egypt. The collected samples were used for the isolation of pigmented actinomycetes. Seventeen actinomycetes were isolated and recognized by morphological and microscopic examinations. The actinomycete EGYl was found to produce two red compounds that were identified as Sharkquinone (1) and SS-228R (2). One of the isolated strains, actinomycete ABS, was found to produce an interesting yellow pigment in a good yield. The structure of the major compound was characterized by spectroscopic methods, including NMR and mass spectrometry data, and identified as Heliomycin (3). The influence of cultural parameters affecting the production of the biomass including different culture media, pH values, temperatures and incubation periods were investigated. Maximum production of secondary metabolites (2.78 g/I) was found in the culture medium of Waksman at initial pH 7.5 for four days at 35°C. The antimicrobial activity of Heliomycin (3) was evaluated against Gram-positive bacteria, Gram-negative bacteria and fungi. |