الفهرس 
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Abstract
In our study, we aimed to solve some problems that face the stem cell transplantation via optimization of the proliferation media and via optimization of the neural &/or glial differentiation media.For optimization of the proliferation media; we first isolated and cultured of AD-MSCs till P3 in media supplemented with 10% FBS. After that proliferation assay was performed and we compared between the effect of 10% FBS and different concentrations of activated P-PRP & A-PRF (0.6, 1.25, 2.5, 5, 10, and 20%) on AD-MSCs proliferation rate. We found that 20% activated P-PRP, 10% activated P-PRP and 20% A-PRF were significantly more potent than 10% FBS.For optimization of the neural &/or glial differentiation media; we isolated and cultured of AD-MSCs from a new lipoaspirate sample till P3 in media supplemented with 20% activated P-PRP instead of FBS. After that, we inducted the neural &/or glial differentiation using different concentrations of CSF for 9 days. On the 9th day, cells were collected for RNA extraction and real time PCR analysis. We found that the expression of MAP2 only significantly up-regulated in (0% CSF + 10% PRP) in comparison to thecontrol group indicating a neurogenic differentiation. At the same time, the expression of GFAP was only significantly up-regulated in (10% CSF +10% PRP) indicating glial differentiation. While the expression of nestin was significantly decreased in all groups in comparison to the control group indicating that cells started to transform from the undifferentiated stage into the differentiated stage.