الفهرس | Only 14 pages are availabe for public view |
Abstract In vitro experiment was carried out to evaluate the effect of gamma rays (20 Gray) on salt resistance of four potato cultivars namely; Lady Rosetta, Diamante, Gold and Santana. Results revealed that gamma-treated Santana cv. plantlets were more resistant to salinity compared to other cultivars with increment by 2.5 times more than untreated ones regarding to fresh weight. Growth improvement was contributed with high concentration of phenolics and free amino acids as well as high density of four and three isoenzymes bands of both peroxidase and polyphenol oxidase, respectively. Genetic marker analysis using ISSR made obvious unique negative and positive bands with different base pairs in mutant plantlets compared to control cultivars according to primer sequence and potato genotype. We can conclude that, using of 20-gray gamma rays dose was more effective tool for induction salt-resistant mutant plantlets of potato cultivars especially Santana cv. and salt-resistant mutants must be re-evaluated on the field experiments |