الفهرس 
Acknowledgement
(Staining of platyhelminths by extract of (Beta vulgarisOnly 14 pages are availabe for public view
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Abstract
Herbal dyes are usually eco-friendly and biodegradable materials. Since natural herbal dyes are cheap and safe for staining of Trematoda and other Cestoda, red beet, henna and china rose can be considered as alternatives to industrial dyes such as carmine.
In wet mount preparations and clinical diagnosis, long-time stability of the stained samples is not the main parameter. However, in cases such as archiving the samples for future studies and training of students, stability of permanently stained samples could be an important issue. from this point of view, more studies are needed to evaluate the color stability of various herbal dyes.
Natural plants are uncostly and innocuous for staining of such as liver flukes such as Fasciola gigantica and hepatica, intestinal parasites Moniezia and Raillietin Spp. The results of this study evidenced that the hibiscus (Hibiscus rosa-sinensis L.) red beet (Beta vulgaris L.) and henna were more efficient than the synthetic dyes.
Using herbal dyes not only gave an excellent results but also provide a safe alternatives to the hazardous, toxic, and potentially carcinogenic chemicals used in usual staining techniques, because it usually used in human nutrition and it is obviously not harmful. Moreover, the specimens stained with these herbal dyes don’t need to be kept in cold or special places but it can be left in the laboratory at room temperature.
In the present study, all three natural herbal dyes preserved their dying ability in a dark bottle for about 1 year at 4 °C. Moreover, they could retain their color in the permanent mount of the stained helminthes for more than a year (until drafting this manuscript).
The stability of permanently stained samples could be considered an important issue in cases such as archiving the samples for future studies and training of students. Moreover, the specimens stained by theses herbal dyes don’t need to be kept in cold or special places but it can be left in the laboratory at room temperature.
So, these herbal dyes possess the potential to replace the conventional dye in wet mount procedure in parasitology field.