الفهرس 
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Abstract
Cytomegalovirus (CMV) is a member of the betaherpesvirinae subfamily. It is the largest among all herpes viruses, with a size of 150-200 nm, containing a linear double stranded DNA molecule in its nucleocapsid. It has been recognized as an important cause of morbidity and mortality in immunocompromised hosts such as patients with acquired immunodeficiency syndrome (AIDS), and recipients of solid organ and stem cell transplants.
Tool Like Receptor 2 (TLR2) is a protein that in humans is encoded by the TLR2 gene. TLR2 is one of the toll-like receptors and plays a role in the immune system.Genetic studies on the TLR2 gene have identified a number of single nucleotide polymorphism ( SNP ) which have been shown to affect host defense and disease progression .TLR 2(SNP) such as (rs5743708), is proved to be associated with tuberculosis, staphylococcal septic shock, heart restenosis, Lyme disease and cytomegalovirus (CMV) disease after liver transplantation. Also it correlates with transplant rejection and higher mortality of chronic hepatitis C patients . Furthermore, bone marrow transplantation which associated with immuncompromised status, can be susceptible for CMV infection which may be associated with genetic variation.
This study aim to estimate the association of single nucleotide polymorphism (SNP) located in TLR2 gene (rs5743708) with the susceptibility of HCMV infection in bone marrow transplantation patients.
The study is consisted of 181 patients after bone marrow transplantation which divided to : -
group (1) 83 patients infected with Cytomegalovirus after bone marrow transplantation.
group (2) 98 patients free of Cytomegalovirus infection after bone marrow transplantation.
where , Collection of blood samples from patients and complete physical diagnosis will be carried out ( Complete Blood Picture, Liver function, Renal function ,RBS) , DNA extraction and purification , Specific Sequence Primer Polymerase Chain Reaction (SSP-PCR) and conformation the results by by the polymerase chain reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP-PCR) method were carried out to investigate the R753Q (rs5743708) polymorphisms in TLR2 gene, Which digested with PstI restriction enzymes , After digestion, 5μl of the products was electrophoresed in ethidium bromide-stained 2.5 % agarose gel. The results were evaluated using UV trans-illuminator and digital photographs were captured.
Data entry and statistical analyses were performed using excel program (Microsoft office 2010), and Statistical Package of Social Sciences (SPSS) version 22.0 (SPSS Inc., Chicago, IL, USA). Continuous variables were expressed as mean ± standard deviation (SD). Testing for deviations of genotypes frequencies from Hardy Weinberg Equilibrium was calculated by applying Hardy-Weinberg model to current data, and the distribution was in Hardy-Weinberg Equilibrium among participants (P values > 0.05).
Among all patients, there is a significant (P ≤ 0.00002) association of TLR2 gene polymorphism and CMV infection susceptibility in bone marrow transplantation patients. However, almost 84% of the patients who were screened for the presence of the TLR2-Arg753Gln were homozygous of G allele(Wild type), only 16% of subjects in the group studied were heterozygous( GA) ,while no patients in either groups was homozygous for the TLR2-Arg753Gln mutation (AA).