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Abstract A total of 112 QMS were collected from cows and subjected to CMT for detection of subclinical mastitis where 85.71% of examined quarter milk samples reacted positively to CMT. SCC was measured and showed that the mean S.E. of SCC was (548.13 x103±24.96) cells / ml of examined quarter milk samples respectively. S. aureus was isolated from 40.62% of positive CMT bovine QMS. E. coli could be isolated from 31.25% of positive CMT bovine QMS. Antagonistic activity of probiotic lactobacilli (L. acidophilus and L. plantarum) against S. aureus isolated from bovine mastitis and E. coli O157 was the objective of this study. Antagonistic effect of probiotic lactobacilli was investigated by well diffusion method, co-aggregation method and modified double layer method. Cell Free Supernatant (CFS) of probiotic lactobacilli in well diffusion method had inhibitory effect. The effect of L. acidophilus on 20 isolated S. aureus strains was 2strains (10%) gave (++++), 2 strains (10%) gave (+++), 6 strains (30%) gave (++) and 10 strains (50%) gave (+) and the effect of L. plantarum was, 2 strain (10%) gave (+++), 8 strains (40%) gave (++) and 10 strains (50%) gave (+). The effect of L. acidophilus on 10 isolated E. coli strains was, 2 strain (20%) gave (++++), 1 strains (10%) gave (+++), 4 strains (40%) gave (++) and 3 strains (30%) gave (+) and the effect of L. plantarum on 10 isolated E. coli O157 strains was, 1 strain (10%) gave (++++), strains (20%) gave (+++), 2 strains (20%) gave (++) and 5 strains (50%) gave (+). The effect of lactobacilli in co-aggregation method showed that the high co-aggregation percentage of L. acidophilus and L. plantarum on 20 strains of S. aureus was 94.63% and 94.61% respectively and the high co-aggregation percentage of L. acidophilus and L. plantarum on 10 strains of E. coli O157 was 92.29% and 90.45% respectively. The effect of L. acidophilus on 20 isolated S. aureus strains by modified double layer was 4 strains (20%) gave (+++), 7 strains (35%) gave (++) and 9 strains (45%) gave (+) and the effect of L. plantarum was, 2 strain (10%) gave (+++), 5 strains (25%) gave (++) and 13 strains (65%) gave (+). The effect of L. acidophilus on 10 isolated E. coli O157 strains was, 1 strain (10%) gave (++++), 2 strains (20%) gave (+++), 3 strains (30%) gave (++) and 4 strains (40%) gave (+) and the effect of L. plantarum on 10 isolated E. coli O157 strains was, 1 strain (10%) gave (+++), 5 strains (50%) gave (++), and 4 strains (40%) gave (+). |