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Abstract The thesis is composed of three parts: Part I: General introduction This part presents a general introduction about cardiovascular diseases, their etiology and the current treatment approach. Different classes of cardiovascular drugs and their mechanisms of action are also discussed. Part II: Development and validation of new analytical methods for simultaneous determination of amlodipine and metoprolol combination This part consists of three chapters: Chapter 1: Review of literature This chapter includes an introduction about the chemical and physicochemical properties of amlodipine and metoprolol and presents a review of literature about the analytical methods recently reported for their assay. Chapter 2: Synchronous fluorescence spectrofluorimetric method for simultaneous determination of amlodipine and metoprolol This chapter includes an introduction about the synchronous fluorescence technique, its types, advantages and applications. This chapter presents the development and validation of a synchronous fluorescence spectrofluorimetric method for the simultaneous determination of amlodipine and metoprolol in their binary mixtures. The developed method was successfully applied for the determination of amlodipine and metoprolol in a laboratory prepared mixture containing all possible excipients present in a tablet dosage form. This method was published in Luminescence, 33 (2018) 364-369. Chapter 3: Green micellar HPLC-fluorescence method for simultaneous determination of metoprolol and amlodipine This chapter presents an introduction about micellar liquid chromatographic technique, its advantages and applications. In this chapter, a green micellar liquid chromatographic method for the separation and determination of metoprolol and amlodipine was developed, optimized, validated and successfully applied for the simultaneous determination of both drugs in their binary mixtures and combined tablet. The developed method was further applied for the determination of metoprolol in spiked human plasma. Assessment of greenness of the method was also performed. This method was published in Microchemical Journal, 147 (2019) 635-642 Part III: Development and validation of new analytical methods for simultaneous determination of atorvastatin and irbesartan combination This part is composed of three chapters: Chapter 1: Review of literature This chapter includes an introduction about the chemical and physicochemical properties of atorvastatin and irbesartan and presents a review of literature about the analytical methods recently reported for their assay. Chapter 2: First derivative synchronous fluorescence spectrofluorimetric method for simultaneous determination of irbesartan and atorvastatin This chapter includes an introduction about the advantages of derivative spectroscopic technique, the combination of synchronous spectrofluorimetry with derivative technique and its applications. This chapter describes the development and validation of a first derivative synchronous fluorescence spectrofluorimetric method for the simultaneous determination of irbesartan and atorvastatin in their binary mixtures. The developed method allowed the determination of irbesartan and atorvastatin in a laboratory prepared mixture containing all possible excipients present in a tablet dosage form. The developed method was further applied for the determination of irbesartan in spiked human plasma. Chapter 3: Green micellar HPLC-fluorescence method for simultaneous determination of irbesartan and atorvastatin In this chapter, a green micellar liquid chromatographic method for the separation and determination of irbesartan and atorvastatin was developed, optimized, validated and successfully applied for the simultaneous determination of both drugs in their binary mixtures and combined tablet. The developed method was further applied for the determination of irbesartan in spiked human plasma. Assessment of greenness of the method was also performed. |