الفهرس 
Genetic alterations in hepatocarcinogenesisOnly 14 pages are availabe for public view
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Abstract
study was designed to evaluate the role of serum leptin level and leptin receptors gene polymorphism in hepatocellular carcinoma and to elucidate their relationship with the clinicopathological features of patients with hepatocellular carcinoma. The subjects of this study were divided into five groups: group I (Control group): Included 10 healthy subjects ranged from 52 to 57 with mean age of 55.0±1.5 years (4 males and 6 females). group II (Chronic hepatitis C): Included 10 patients with chronic hepatitis C ranged from 50 to 60 with mean age of 56.3 ±3.3 years (6 males and 4 females). group III (Non- alcoholic steatohepatitis): Included 10 patients with non alcoholic steatohepatitis ranged from 50 to 62 with mean age of 57.3 ±3.5 years (3 males and 7 females). group IV (Liver cirrhosis): Included 10 patients with liver cirrhosis ranged from 52 to 67 with mean age of 55.3 ±4.7 years (4 males and 6females). group V ( Hepatocellular carcinoma ): Included 20 patients with hepatocellular carcinoma ranged from 50.0 to 69.0 with mean age of 59.8 ±5.1 years (15 males and 5 females). All cases were subjected to the following: 1) Full History taking. 2) Complete clinical examination 3) Abdominal ultrasonography 4) Liver biopsy to confirm the diagnosis whenever possible 5) Routine laboratory investigations: * Liver function tests including: Total and direct serum bilirubin , ALT, AST, ALP, total serum proteins, albumin, prothrombin activity. * Viral hepatitis markers. * Serum alpha-fetoprotein . 6) Specific laboratory investigations: * Serum leptin level. * Leptin receptors gene polymorphism by (RFLP method). The results of the present study showed the following: Serum leptin level was not significantly different in HCV, patients as compared to controls. it was significantly increased in NASH patients as compared to control .It was significantly decreased in cirrhotic , and HCC patients as compared to control. Serum leptin was not increased with the degree of deterioration of functional liver status in terms of advanced Child-Pugh class. There was significant positive correlation between serum leptin level and prothrombin activity but there were significant negative correlations between serum leptin level and ALT, AST and child score in chronic liver diseases . However, no significant correlation was found between serum leptin level and age, BMI, ALP and albumin in chronic liver diseases . In HCC patients, there were significant negative correlations between serum leptin level and both tumor grade and tumer size. However, no significant correlation was found between serum leptin level and age,TNM stage, Child score. LEPR Gln223Arg, GG and GA genotypes were found in all studied groups but LEPR Gln223Arg, AA genotype was found in NASH, HCC and control. LEPR Gln223Arg GG genotype was represented in 50% of controls, 30% of chronic hepatitis C patients, 50% of NASH patients, 20% of cirrhotic patients and 60% of HCC patients. LEPR Gln223Arg GA genotype was represented in 40% of controls, 70% of chronic hepatitis C patients, 40% of NASH patients, 80% of cirrhotic patients and 35% of HCC patients. LEPR Gln223Arg AA genotype was represented in 10% of controls, 10% of NASH patients, and 5% of HCC patients. LEPR Gln223Arg GA genotype is associated with the risk of HCC. In HCC patients, there were no relationship between the clinicopathological features and LEPR Gln223Arg genotypes.