الفهرس 
Genomic InstabilityOnly 14 pages are availabe for public view
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Abstract
Genomic instability is an enhanced tendency for the genome to acquire mutations,ranging from changes to the nucleotide sequence to chromosomal gain, rearrangements or loss. Genomic instability plays critical roles in both cancer initiation and progression. The aim of the present study was assessed the genomic instability in the pathogenesis of experimental model of mammary adenocarcinoma. Concomitantly, this study was evaluated the antitumor potential of anthocyanin.Anthocyanin was extracted from plum and concentration was determined. Sevent
albino mice were divided equally into; group I(Negative control); group II(Anthocyanins); group III (Methotrexate (MTX) & Anthocyanins); group IV (Ehrlich
solid tumor bearing); group V (Ehrlich & MTX); group VI (Ehrlich &Anthocyanins);group VII (Ehrlich & MTX & Anthocyanins). The tumor weight and volume were evaluated. Serum ALT, AST activities and urea, creatinine levels and arylesterase activity were determined. Nuclear PARP, cytoplasmic GST activities, DNA damage were
evaluated in solid tumor. Genomic instability was assessed by Random amplified polymorphic DNA (RAPD) in solid tumor.Serum arylesterase activity was lower in all mice induced tumor and treated with anthocyanins, MTX or combined with both. PARP activity was elevated and GST activity was low in tumor treated with anthocyanins, MTX or combined with both as compared with untreated tumor. DNA damage was increased in mice with solid tumor
and treated with MTX or MTX & anthocyanins as compared with untreated tumor mice. There was in significant change in genomic instability in tumor mice treated with anthocyanins.Anthocyanins have been reported to exert positive effects in the treatment of mammary adenocarcinoma. Anthocyanins combined with MTX may also ameliorate the tumor by enhancing cell death pathway driven by DNA damage.