الفهرس 
General Introduction
LetrozoleOnly 14 pages are availabe for public view
 |  | from | 193 |  |  |
| | | from | 193 | | |
Abstract
Part I: General Introduction
It includes a general introduction on Nitriles including size and shape of Nitrile group, chemical and physical properties, metabolism and stability. It shows several examples of pharmaceuticals in market with nitrile participating in their mechanism of action.
Part II: Literature Review
It includes a detailed review on the studied drugs, Letrozole and Vilazodone HCl. This review explains chemical and physical drug properties, pharmacology, structure activity relationship, pharmacokinetics and different analytical methods reported in literature.
Part III: Spectroscopic Analysis of Letrozole
This part consists of two sections:
Section A: A Spectrophotometric Determination of Letrozole, Tramadol and Metoclopramide in Rat Brain Tissue Homogenates Using Dual-wavelength in Ratio Spectra Method:
In this section, DWRS method was applied for the simultaneous determination of Letrozole, Tramadol and Metoclopramide. For Letrozole, the zero order spectra were divided by zero order spectrum of 27 μg/ml Metoclopramide and the amplitude difference of the ratio spectra between 239 and 243 nm was utilized for determining concentration without interference. For Tramadol, the zero order spectra were divided by zero order spectrum of 7μg/ml Metoclopramide and the amplitude difference of the ratio spectra between 272 and 280 nm was utilized for determining concentration without interference. Metoclopramide was directly determined from zero order spectra at 310 nm. The method was validated as per ICH guidelines. It was successfully applied for the determination of the three drugs in spiked rat brain tissue homogenates using protein precipitation technique and QuEChERS extraction technique, showing good recovery percentages.
Section B: Spectrofluorimetric Determination of Letrozole in Tablets, Plasma and Rat Brain Tissue: Application to Alkaline Degradation Kinetics:
This method utilizes the native fluorescent nature of Letrozole. It measures RFI at 590 nm upon excitation at 239 nm. The method utilizes a new solvent mixture of water and Acetonitrile (70:30%, v/v). It has been successfully applied to tablet formulations and biological samples (plasma and brain tissue) using a simple Liquid Liquid Extraction (LLE) technique. It was further applied to study alkaline degradation kinetics of Letrozole. Reaction rate constant and half-life time were calculated.
Part IV: Potentiometric Analysis of Vilazodone
This part proposes two sensors for the potentiometric determination of Vilazodone. Sensor 1 is PVC based membrane plasticized with dioctyl phthalate and ion association complex of Vilazodone-Molybdate as sensing element. Sensor 2 is Nanocomposite carbon paste electrode obtained by mixing Graphene nanoplatelets and ion pair using paraffin oil. Nernestian response was achieved with slope values 59.89 and 59.91 mV/decade and LOD 9.8 x 10-8 and 1 x 10-8 M for sensors 1 and 2; respectively. Sensor 2 showed several advantages over sensor 1. Both electrodes were used for analysis of Vilazodone in pure solutions, spiked human plasma and spiked formula milk samples with good recovery percentages.