الفهرس | Only 14 pages are availabe for public view |
Abstract Industrial areas in Egypt has become one of the major routes of some wastes production as it discharges a large amount of petroleum hydrocarbons which possess severe hazardous effects on the surrounding environment. Aromatic hydrocarbon is one of the most toxic organic pollutants, among this organic hydrocarbons is anthracene and carbazole which they highly carcinogenic. The environmental threat of those aromatic hydrocarbons to humans due to exposure through industrial effluent discharge and other combustion activities into the environment beside expensive nature of the physical and chemical remediation techniques initiates this research. In this study anthracene and carbazole were degraded by bacteria isolated from industrial waste water areas and degradation was carried out in liquid minimal salt media (MSM). From the fourteen bacterial strains isolated only three isolates were selected that show high potency for degrading anthracene and carbazole. Isolates GH1, GH17 and GH21 were identified biochemically and genetically by 16S rRNA sequence as Lysinibacillus fusiform, Bacillus subtilis and Serratia liquefaciens, resepectively. Some environmental factors which effect on their degradation were also studied in this research to optimize its degradation process. The intermediates formed during anthracene and carbazole degradation were identified by GC-MS spectroscopic analysis, which revealed anthracene degradation pathway. |