الفهرس 
Pediatric Acute Lymphoblastic LeukemiaOnly 14 pages are availabe for public view
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Abstract
Introduction: The presence or absence of specific genetic abnormalities present in leukemic blast cells plays an essential role in determining the outcome and the treatment plan. Among the known prognostic lesions in ALL is Hyperdiploidy with specific extra chromosomes or an ETV6-RUNX1 rearrangement which were found to promise an excellent outcome.
Aim: The aim of this work is to detect intrachromosomal amplification of RUNX1 gene on chromosome 21(iAMP21) in pediatric -ALL by fluorescence in situ hybridization (FISH) technique and to correlate its presence with the other known prognostic factors.
Patients and methods: The present study was carried on thirty newly diagnosed or relapsed pediatric ALL patients. They were attending Hematology Oncology unit of pediatric Ain Shams University Hospital. Their ages ranged from 1.33 to 16 years with a mean value age of 7.76 years ±5.11. They were 23 males and 7 females with a male to female ratio of 3.2:1. To reach diagnosis and classification, all patients were subjected to the following.
Results: The study was carried out on thirty ALL patients: 25newly diagnosed and 5 relapsed cases, 23/30 (76.7%) were males and 7/30 (23.3%) were females with male to female ratio 3.2 to 1. Their age ranged from 1.33 to 16 years with a mean value of 7.76 years ± 5.11
Conclusion: The occurrence of amplification in higher percentage (36.7%) among our Egyptian ALL patients than recorded before (5%).
Keywords: Intrachromosomal amplification of RUNX1 gene (iAMP21) on chromosome 21 in pediatric acute lymphoblastic leukemia