الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
 |  | from | 184 |  |  |
| | | from | 184 | | |
Abstract
The steadily increasing applications of radiation in clinical practice, industrial and agricultural activities and an enormous challenge for the biological and medical safety make us focus on the harmful effect of ionizing radiation. Therefore, this study was designed to show the beneficial effects of silymarin at dose (70mg/Kg b.wt.) and MSCs (1.4x107 cells) against the harmful biological effects of gamma radiation after one and three weeks of exposure. The results of the present study indicated that gamma radiation exposure caused the following alterations in comparison with the control group: A significant decrease in RBCs, WBCs and platelets count accompanied by significant decrease in Hb content as well as Hct%, MCH and MCV values. Serum and liver total protein (TP) content were significantly decreased accompanied by a significant decrease in serum albumin, globulin and A/G ratio that seemed to be highest after three weeks of exposure. Total Lipid (TL), total cholesterol (TC) and triglycerides (TG) levels were significantly elevated in serum and liver, an effect that seemed more evident with three week treatment. In contrast, serum high density lipoproteins (HDL-C) concentration was decreased in parallel with elevation in serum low density lipoprotein (LDL-C) and very low density lipoprotein (VLDL-C) concentration. Additionally, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were significantly increased, in combination with significant reduction in these enzymes in the liver tissue. Serum bilirubin level was significantly increased. Liver content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) level were significantly increased and this goes along with significant decrease in reduced glutathione (GSH) content, total antioxidant capacity (TAC), catalase (CAT), glutathione-S-transferase (GST), superoxide dismutase (SOD) and glucose-6-phosphate dehydrogenase (G-6-PDase) activity. Besides, molecular analysis of liver tissues of male rats revealed that gamma radiation exposure induced mutation in liver DNA profile with the two primers OP-B10, OP-B14. In contrast, intake of silymarin and transplantation of mesenchymal stem cells seemed to control harmful effect of gamma radiation exposure through inhibiting the increase in lipid peroxidation and hydrogen peroxide and enhancing the antioxidant enzyme activities besides, a reduction in the lipid profile, and hepatic enzymes. Accordingly, it can conclude that intake of silymarin has a hepatoprotctive effect while, MSCs have therapeutic effects against gamma radiation exposure.