الفهرس | Only 14 pages are availabe for public view |
Abstract Atherosclerotic cardiovascular disease, a chronic inflammatory disease considered as the leading cause of death worldwide. MicroRNAs have been shown to be associated with physiological and pathological conditions as inflammation and cardiovascular disease. On the basis of the communication among microRNAs, inflammation, and atherosclerosis, we hypothesized that inflammation-related miRNAs, miRNA-155, could play a role in the progress of atherosclerosis. In the present study, we aimed to determine the levels of circulating miRNA-155 in patients with chronic stable angina and to study the impact of miRNA-155 on the severity and extent of coronary artery disease. MicroRNA was extracted and assessed from plasma of 50 subjects (20 controls and 30 patients with chronic stable angina) using quantitative reverse-transcription PCR (RTPCR). Levels were compared in the two groups and correlated with Gensini score in the group with chronic stable angina (CSA). We found that, Plasma levels of miRNA-155 were significantly lower in CSA patients (0.59±0.48, 1.94±0.76, in patients and control group respectively. P<0.001). The expression of miRNA-155 correlated negatively with Gensini scores (P<0.001), total cholesterol (P <0.001), LDLc (P= 0.002) and triglycerides (P= 0.03). There was a significant difference in miRNA-155 levels among the quartiles of the CSA group (P < 0.001) indicating an inverse correlation between miRNA- 155 and the extent of coronary artery disease. ROC curve showed that miRNA-155 sensitivity and specificity for the prediction of severity of atherosclerosis were 86.67% and 80% respectively. |