الفهرس 
List of ContentsOnly 14 pages are availabe for public view
 |  | from | 77 |  |  |
| | | from | 77 | | |
Abstract
Aim of study: this study was designed to evaluate, in vitro, different therapeutic effects (antimicrobial, anti inflammatory and biocompatibility) of GlycyIThizin for use in root canal treatment. Materials and Methods: In this study, the herbal medicinal extract used for tht; experimental purpose was Glycy rrhizin. The antimicrobial evaluation of Glycyrrhizin against the most resistance microorganisms in root canal treatment (E. faecalis and candida albicans) was performed using microdilution test for measuring the minimum inhibitory concentration (MIC) to determine the effective concentration, to be used in the anti inflam.matory and biocompatibility assays. Cyclooxygenase Inhibitory assay and Lipoxygenase inhibitory assay were used to evaluate anti-inflammatory activity of glycyrrhizin and WI-38 cell line was used to evaluate the biocompatibility of glycyrrhizi1! Compared to the most comm. used irrigations and medications in endodontics [2.5% Sodium hypochlorite NaOCL, 2% Chlorhexidine (CID<), so_lution, Calcium hydroxide Paste, Chlorhexidine CHX gel, Mix ofCa(OH)2 / CID<, Lederm ix, Triple antibiotic paste (TAP) and Double antibiotic paste (DAP)]. Results: broth microdilutiontest showed that, the minimal bactericidal concentration of glycyrrhizin against E. fecalies was 12.5 μg/ml while the MIC against C. albicans was 23.2 μg/ml. the result of anti-inflammatory assays showed that, for both Cyclooxygenase and Lipoxygenase assays, glycyrrhizin had significantly (p < 0.005) higher anti-inflammatory action than that of ledermix. The biocompatibility of glycyrrhizin was found to be highly significant (p < 0.005) compared to the all tested root canal irrigations and medications, as indicated by MTT assay. Conclusion: within the limitation of this study, it was found that glycyrrhizin could be considered to have good potential as a root canal irrigant or medicament. The commonly used irrigations andmedications should be used with caution in endodontic thereby, since the drugs act not only against microorganism as well as in cell of the perradicular tissue. Further laboratory andclinical investigationshouldbe carried out to validate finding of beneficial of glycyrrhizin as intracanal medicament or asanyother endodontic materials.