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العنوان
Studies on some virulence genes of klebsiella pneumoniae isolated from clinically diseased chickens /
المؤلف
Khattab, Rania Hosny Abdel-Aziz.
هيئة الاعداد
باحث / رانيا حسني عبدالعزيز خطاب
مشرف / جمال عبدالجابر محمد يونس
مشرف / أحمد محمد عبدالجواد الجمل
مشرف / أمل عبدالستار إبراهيم
مناقش / أحمد عمر شفيق
مناقش / كامل ابراهيم ابوالعزم
الموضوع
Virulence (Microbiology) Klebsiella. Virulence - genetics.
تاريخ النشر
2017.
عدد الصفحات
127 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
1/1/2017
مكان الإجازة
جامعة المنصورة - كلية الطب البيطرى - Bacteriology, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

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from 127

Abstract

, 30 (15%) Klebsiella isolates differentiated into K. pneumoniae 73.33 % (22/30) and K.oxytoca 26.67 % (8/30) were recovered from 200 tissue samples examined conventionally and confirmed by PCR using genus specific primer sequences (gyrA) The isolation rate of Klebsiella spp. was 46.67%, 20%, 20% and 13.33 % from lungs, liver, spleen and heart respectively. The isolation rate of Klebsiella from lungs was higher than the other organs In our study hemolytic activity of Klebsiella detected in 40% (12/30 The entire 30 isolates were screened by PCR to identify the mag A and rmp A genes by using specific primer sequences. Out of the tested isolates, 46.67% (14/30) were positive for rmp A gene (Photograph 6) and 53.33% (16/30) were positive for mag A gene. In this study, hyperviscosity (HV) was detected in 16 out 30 Klebsiella isolates. HV phenotype was highly correlated with the presence of the rmpA gene. Of 16 mucoid isolates, 81.25% (13/16) were rmpA gene positive, and 18.75% (3/16) were rmpA gene negative. Among non-mucoid (14) isolates, 92.86% (13/14) were negative for the rmpA gene and 7.14% (1/14) were rmpA positive. Klebsiella isolates were tested against 12 antimicrobial agents using the Kirby-Bauer disk diffusion assay. All of the isolates were resistant to amoxicillin (AX). The resistance rates for cephalosporin were 86.67% for cefotaxime, 70% for cefapime 66.67% for ceftriaxone.