الفهرس 
ACKNOWLEDGMENTOnly 14 pages are availabe for public view
 |  | from | 135 |  |  |
| | | from | 135 | | |
Abstract
This study was conducted on 30 white albino rats where an osteotomy (1mm diameter and 1 mm deep) was made under constant sterile saline irrigation with an inverted cone bur to prepare undercuts to retain the material on both sides of the mandible
The procedure was performed on both sides of the mandible, where the MM-MTA was placed in the right side of the mandible and the conventional Grey ProRoot MTA was placed in the left side.
General anaesthesia was performed through intra-muscular injection of ketamine hydrochloride (Ketlar 0.5%) v, 20 mg / kg body weight, and xylazinevi (1 mg / kg body weight). After routine disinfection, and preparation of surgical field by betadine, an extra-oral submandibular incision was performed at the right inferior border to raise a full thickness skin flap. The masseter muscle was incised and dissected from periosteum to expose the body of the mandible. By using low– speed round bur number 2, with copious irrigation, a surgical bony defect of ≈ 1 mm. in diameter was done.
The materials were mixed, delivered, and compacted into the prepared osteotomies according to the manufactures recommendation. After implantation, the soft tissues were held in place with a 6-0 polyglycolic acid resorbable suture material, and a fluid tight seal was obtained with histoacryl.
Fifteen rats were sacrified after one week to evaluate the short term inflammatory response and the other fifteen rats were sacrified after three weeks to evaluate the long term inflammatory response. Specimens were fixed with 10 percent buffered formaline for 48 hours, dehydrated in ascending concentrations of ethyl alcohol (from 50 to 100), infiltrated with xylene and
77
embedded in paraffin wax. Serial sections of the prepared paraffin blocks were cut by microtome to (5mm) thickness. The specimens were stained by Hematoxyline and eosin (H&E) and Masson’s trichrome stain (T.C).
Specimens were observed under light microscope for the presence of inflammatory cells and were categorized according to an ordinal scale. After three weeks histomorphometric analysis was achieved to calculate the bone volume.
The findings of this study concluded that MM-MTA is considered to be less biocompatible than Grey pro root MTA with a statistically significant result .Both materials are considered to have the ability to form bone with no statistical difference between both materials on rat mandibular tissue.