الفهرس | Only 14 pages are availabe for public view |
Abstract Studies were made on 39 different microbial cultures (15 fungi and 24 bacteria), Ps. fluorescens was found to be the most active organism. The highest prednisolone yield (63.4 %) was obtained by using 5 mg of cortisoV50 ml medium and addition of0.6 rnVl of H202 at optimum conditions, transformation medium (beef extract, 3; peptone, 5 and glucose, 10 g/1), pH 6.5, temperature 30°C, culture age 48 hand agitation speed 200 rpm. Ca-alginate, polyacrylamide and poly-HEMA were used to transfonn cortisol. Poly-HEMA immobilized cells was chosen, the prednisolone yield was increased to 84.3 %by the repeated use of the immobilized cells for 7 cycles. Prednisolone yield reached to its maximum (96.8 . %) by the repeated use of the immobilized cells for 9 cycles by using 2L fennentor. Isolation and identification of the transfonnation products were carried out by using modem teclmiques. |