الفهرس 
Low level laser therapy (LLLT)Only 14 pages are availabe for public view
 |  | from | 153 |  |  |
| | | from | 153 | | |
Abstract
Aim: This study was designed to evaluate the effect of low level laser therapy (LLLT) on
odontoblast-like cells derived from human bone marrow mesenchymal stem cells (hBMMSCs).
Material and Methods: 40 flasks of odontoblast-like cells were obtained from 10
samples of h-BMMSCs and divided into 2 groups; control group (A) and diode laser
(GaAlAs) irradiated group (B). Each group was further subdivided into 2 subgroups,
where subgroups A1, B1 and A2, B2 were examined after 3 and 14 days after
odontoblastic differentiation respectively. Each subgroup was examined by Inverted
Phase Contrast Light Microscope to examine cell count, viability, Alizarin Red
Stained (ARS) mineralized matrix. Real Time- Polymerase Chain Reaction (RT-PCR)
was performed for each group to detect, the amount of gene expression of Dentin
Matrix Protein 1 (DMP1) and Alkaline Phosphatase (ALP). The data of cell count and
RT-PCR were statistically analyzed.
Results: Regarding time of examination, cell count and viability statistical results showed
a non-significant increase in 3 days control and irradiated subgroups (A1 and B1)
compared to their corresponding control and irradiated 14 days subgroups (A2 and
B2). DMP1 gene expression statistical results were significantly increased in 14 days
control and irradiated subgroups (A2 and B2) than 3 days subgroups (A1 and B1).
ALP gene expression results in the control subgroup at 14 days (A2) were
significantly decreased than at 3 days in control (A1) subgroups and significantly
increased in irradiated at 14 days (B2) than at 3 days (B1) subgroups. ARS results
showed more calcific deposits in 14 days control and irradiated subgroups (A2 and
B2). Regarding to irradiation, there was non-significant increase in cell count and
viability results in irradiated subgroups at 3 and 14 days (B1 and B2) in comparison
to their controls (A1 and A2). DMP1 and ALP gene expression results were increased
significantly in irradiated subgroups (B2 and B1) than their controls (A2 and A1)
respectively. ARS sections showed increased calcific deposits in irradiated subgroups
(B2 and B1) in comparison to their controls (A2 and A1).
Conclusion: LLLT enhanced DMP1 and ALP gene expression, cell proliferation and
matrix mineralization in odontoblast-like cells derived from h-BMMSCs.
Keywords: Odontoblast-like cells, BMMSCs, Diode laser, RT-PCR, DMP1, ALP.