الفهرس 
Introduction and objectivesOnly 14 pages are availabe for public view
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Abstract
Purpose: to evaluate the vital use of the following dyes to stain the anterior capsule: Bismark
Brown 1%, Brilliant Cresyl Blue 1%, Crystal Violet 0.1%, Flourescein 1%, lndocyanine Green 1%,
Methylene Blue
1%, Methylene Green 1%, Neutral red 1%, Nile Blue A 1%, Rhodamine 6G 1%, Rose Bengal 0.5% and
Trypan Blue 0.06% to be tested for staining capability as well as endothelial toxicity.
Methods: the experiment was preformed on 40 New Zealand albino rabbits anaesthetized by an
intravenous combination of Ketamine and Xylacine. The dye is the injected on the capsule surface
after filling the anterior chamber with air ihrough a paracentesis and spread with the cannula
(27#). Extra dye is washed out as we ll as air and replaced with methyl-cellulose 2%. The anterior
chamber is then opened with a 2.75-mm keratome through which the capsulorhexis is formed using
cyctotome and capsulorhexis forceps. The animal is sacrificed one hour later using intravenous T61,
0.5 ml/Kg.
Results: Best visibility with least endothelial toxicity were achieved with Methylene Green 0.5%
and 1%, Rose Bengal 0.1%, Gentian Violet 0.05%, Trypan Blue 0.06% and Brilliant Cresyl Blue 0.5%.
The most toxic dye was the methylene blue, while the least were the fluorescein and ICG.
Fluorescein 1% and Nile Blue A 1% didn’t stain the lens capsule enough to perform the capsulotomy.
Histopathology confirmed the photometry results. Vitreous leakage was not detected by photometry of
the vitreous for the four dyes tested.
Conclusion: Clinical trials should be directed towards Gentian Violet, Rose Bengal and Methylene
Green, being of low endothelial toxicity to confirm their safety n vivo.