الفهرس | Only 14 pages are availabe for public view |
Abstract Based on the results obtained from the present study it could be concluded that: The main genotype responsible for E. granulosus human infection in Egypt is G6, the camel strain. PCR is very sensitive and specific in the diagnosis of CE either using patients’ HCF or sera. Sequencing following PCR confirmed that the human infection in the present study were caused by G6 genotype and showed 100 % identity and homology with the G6 Japanese strain and the Egyptian G6 strain on GenBank. Examining cysts of different animals (camels and sheep), revealed that camels had true fertile hydatid cyst and considered to be the main animal reservoir of CE in Egypt, while none of the examined sheep cyst were fertile; had protoscolices. SDS-PAGE analysis of the protein profiles of human and camel were similar with minor differences mainly in high molecular weight protein bands of 164 , 130 KDa. EITB using crude HCF antigen against the studied group’s human sera revealed that the 48-kDa and 12-kDa protein bands had the highest reactivity (100%) in the CE cases. Also, the 35, 75, 56 and 25 KDa bands were recognized by 66.7%, 23.3%, 10% and 33.3%, respectively by CE sera. The 110, 80 and 65 KDa bands were not recognized by both CE cases and control groups’ sera. All the reactive protein bands were absent in patients sera after treatment except the 35KDa, which make it less sensitive to be used to follow up and ensure the success of treatment. So, the crude HCF antigen prepared from the G6 E. granulosus strain has many antigenic proteins but specifically the 12 KDa band were recognized by anti- E. granulosus IgG and was absent in all control sera and also absent in patient sera after treatment making it the most sensitive and specific antigenic band in diagnosis of CE. |