الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was planned to investigate the efficacy of using A.hydrophila and Ps.fluorescence formalized killed vaccine and non specific immune stimulant feed additives ( garlic and yeast) in African catfish (Claris garpinus). The studies were carried out on 400 apparently healthy catfish collected from Manzala private fish farm. The results obtained from this investigation recorded that catfish collected for the present study had a natural antibodies specific to A.hydrophila and Ps.fluorescence bacteria which detected by ELISA technique at zero day of experiment as 0.22±0.004 and 0.20±0.009 optical density (O.D) respectively. The antibody level gradually increased reaching its high peak at 21 days post vaccination then gradually decreased after challenge reaching its basal level at 42 days post vaccination while the control groups remain constant through out the experiment as in zero day. Intraperitoneally vaccinated catfish with A.hydrophila, Ps.fluorescence and bivalent formalized killed vaccine show a high antibody titer throughout the experiment when compared with that vaccinated by oral route and immersion route. The obtained results revealed that the RPS of catfish vaccinated intraperitoneally by A.hydrophila, Ps.fluorescence formalized killed vaccine and bivalent vaccine were 72.2 ,68.7 and 62.5% respectively. while RPS of catfish vaccinated orally by A.hydrophila, Ps.fluorescence formalized killed vaccine and bivalent vaccine were 66.6 ,56.2 and 56.2 respectively. RPS of Cat fish vaccinated by immersion with A.hydrophila, Ps.fluorescence formalized killed vaccine and bivalent vaccine were 61.1, 50 and 50% respectively. The results indicated that lysozyme activity, NBT and blood parameter activity were enhanced with increase garlic and yeast levels compared with control groups. The relative percent of survival was 12.5, 25 and 37.5 % for fish fed on diet contain 1, 1.5 and 2% garlic respectively. The relative percent of survival was 12.5, 18.7 and 31.2 % for fish fed on diet contain 1, 1.5 and 2% yeast respectively. |