الفهرس 
Historical AspectsOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
Microsporidia are recognized as parasitic infectious agents worldwide in both developed and developing countries. Microsporidiosis was reported in both immune compromised and immune competent patients. The present work aimed to clarify the role of microsporidia as a cause of parasitic diarrhea and their genotype patterns in symptomatic and asymptomatic immunocompromised patients. Stool samples were collected from 323 patients in Ain Shams University hospitals. Patients were divided into 2 groups; immune suppressed patients (group I) (n=173) and immune competent individuals (group II) (n=150). Stool smears were stained by modified trichrome and modified Ziehl-Neelsen stains where 45 samples were microsporidia positive, 25 and 20 stool samples belonging to group I and II, respectively. The microsporidia positive stool samples were subjected to PCR amplification (nested and RFLP-PCR). Both nested and RFLP-PCR detected 44/45 samples with 97.8% sensitivity, 100% specificity.
Animal contact was noted in 4% and 40% of microsporidia positive patients in group I and group II, respectively. Ent. bieneusi showed statistically significant difference with animal contact (p value=0.03). Different abdominal symptoms were manifested in 60% and 50% of microsporidia positive patients in group I and group II, respectively, with high statistical difference (p < 0.01) with different microsporidia species detected. There was no statistically significant difference regarding immune status and associated microsporidial infection. There was high statistical significant difference (p value < 0.01) for infection with Enc. intestinalis in renal transplant recepient. Sequencing of the internal tanscribed spacer of rDNA of five samples demonstrated the Ent. bieneusi anthroponotic genotype B with 100% identity to published blast sequence and the zoonotic potential for genotypes D and K with identity of 100% for both genotypes, in addition to a case with Enc. intestinalis with 99% identity to published blast sequence.