الفهرس 
: REVIEW OF LITERATUREOnly 14 pages are availabe for public view
 |  | from | 209 |  |  |
| | | from | 209 | | |
Abstract
In this study, 130 blood samples were collected from cows Bos indicus in Port Said governorate in the Delta. Samples examined by microscopy and resulted that 16 animals 13% infected with Babesia spp., 44 animals 33.8% infected with Theileria spp. and 67 animals 54.5% infected with Anaplasma spp. Using PCR reactions on some randomly selected samples, results were 23% infected with Babesia spp., 68.8% infected with Theileriaannulata , 88.4% infected with Anaplasma spp. and 59.5% infected with Rickettsia spp. Then, tams-1 gene, obtained from PCR with Th5-2, was partially sequenced and revealed the presence of two different tams-1 sequences in Egyptian isolates of Theileriaannulata put under the accession numbers KF765518 and KF765519 in gene bank. Based on microscopy data, seasonal variations indicate that the infection with Theileria was 71.1% in summer, 15.8% in spring, 10.5% in winter and 2.6% in fall. But with PCR results 77.3% in summer, 9.1 in spring and winter and 4.5% in fall. By statistical analysis of microscopy and PCR data, PCR seems to be more sensitive for parasites detection.