الفهرس 
General experimental proceduresOnly 14 pages are availabe for public view
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Abstract
The current thesis is divided into two studies as a part of our ongoing
project. First we investigated the species visnaga with the objective to isolate,
purify and structure elucidation the compounds as a part of our ongoing
project for bioactivity direct fractionation of medicinal plants.
Second to investigate the phytochemical, cytotoxic and antimicrobial
properties exhibited by the bioactive compounds extracted from colonies of
Egyptian soft coral Sarcophyton trocheliophorum.
The present research work aims at:-
1. Isolation and purification of the bioactive compound(s).
2. Elucidation of the structure by mass spectrometry and details 1D and
2D NMR spectroscopy.
3. Screening the biological activity of the soft coral crude extracts using
different bioassays.
4. Bioassay guided fractionation for isolating active ingredients emphases
on cytotoxic and antimicrobial activity.
The present study covered the following points:
Chapter 1: Introduction and literature review
1st part: includes review of literature about natural products which
contribute significantly to primary health care worldwide with focus on plant
Ammi visnaga.
2nd part: includes review of literature about marine as natural sources
and description of Soft corals S. trocheliophorum and their natural existence.
Chapter 2: Experimental work
1st Part: Describes the apparatus and methods used for isolation,
purification and identification of compounds from plant A. visnaga.
2nd Part: Includes extraction of marine materials used in the present
study (S. trocheliophorum), and describes the methods used to test the cytotoxic and antimicrobial activities by using Brine shrimp lethality bioassay
and growth inhibition activity by turbidity measurement method respectively.
Chapter 3: Results and discussion
1st Part: Our investigation on plant A. visnaga leads to isolation of one
chromone glycoside, along with two furano chromone and two flavonoid
compounds. The structure elucidated by IR, one-and two-dimensional NMR,
high-resolution mass spectrometry and comparison with spectroscopic data of
closely related structures.
2nd Part: In the present study on the colonies of soft coral (S.
trocheliophorum) cytotoxicity was evaluated using the brine shrimp lethality
bioassay and antibacterial activity was evaluated against the pathogenic
bacteria Bacillus cereus, Staphylococcus aureus, Salmonella typhi,
Escherichia coli and Pseudomonas aeruginosa using the disk diffusion assay.
The hexane extracts were more cytotoxic than the ethyl acetate extracts. Two
fractions from the hexane extract and one from the ethyl acetate extract
showed significant cytotoxic activity against brine shrimp nauplii. Moreover,
two fractions of the hexane extract demonstrated good activity against S.
aureus and B. cereus in zone of growth inhibition assays. One fraction of the
ethyl acetate exhibited strong activity against E. coli and S. typhi. The results
indicate that extracts from the studied soft coral contain bioactive compounds
that could potentially prove useful in the development of anti-cancer drug
leads and antibacterial agents.