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Abstract Agricultural and food industries in the Arab world represent a large proportion of waste was estimated at18.14 million tons per year and represent remnants of fruit and vegetables many facture about 6.14% of this amount. Waste in the food industry is characterized by a high ratio of product-specific waste. Processing of fruits, vegetables, and oilseeds may results in high amounts of waste materials such as peels, seeds, stones and oilseed meals. Disposal of these materials usually represents a problem that is further aggravated by legal restrictions. Plant waste is prone to microbial spoilage; therefore, drying is necessary before further exploitation. The cost of drying, storage, and transport poses additional economical limitations to waste utilization. Therefore, agro-industrial waste often is utilized as feed or fertilizer. However, demand for feed or fertilizer varies and depends on agricultural production. Moreover, valuable nutrients contained in agro-industrial wastes are lost. Thus new aspects concerning the use of these wastes as by-products for further exploitation on the production of food additives or supplements with high nutritional value have gained increasing interest because these are high-value products and their recovery may be economically attractive. Therefore, the objective of this work is to analyze some plant by-products including potatoes and prickly pear peels that constitute to waste for their nutraceutical values as food ingredient thus reducing its contribution to environmental pollution. Also, utilization of such by-products for their recovery of dietary fibers, phenolics and antioxidants in the production of snacks such crackers will be in the scope of this study. To achieve these aims, the plan of study was prepared as follow: 1) Potato peel powder (PPP), and Prickly pear peel powder (PPPP) were prepared and their chemical and physical properties as well as antioxidant activity were determined. 2) The rheological properties for both wheat flour control sample and samples with additions of selected food by-products were determined by using of farinograph and extensograph tests, and 3) Cracker samples were prepared. Formulation of the Cracker is applied as follow: wheat flour, 100 g; shortening, 9.64 g; salt, 2.02 g; sodium bicarbonate,0.16 g; yeast, 3.48 g and water 50 g. Yeast was mixed with water (25 0C) to form a suspension, to which the other ingredients were then added and kneaded to form smooth dough. Substitution of wheat flour with prickly pear peel powder (PPPP) and potato peel powder (PPP) were conducted based on 5% of the weight of the wheat flour. The dough was later proofed for 2 hours in a proofer, followed by sheeting to 1.0mm thickness using a dough sheeter. The dough was then cut into squares measuring 3cm x 3cm and ‘docked’ prior to baking at 170 0C for 15 min. The sensory evaluation and antioxidants content for both cracker control samples and samples with additions of selected food by-products were determined by using of the specific methods. The obtained data are summarized as follow: I. Antioxidant activities and total phenolics in selected food by-products I.1 Antioxidant activities of selected food by-products The by-products powder showed considerable differences in antioxidant activity (AA = 71.05 - 83.22 %) when it was calculated by the four different methods used in this study. potato peels powder (PPP) showed strong activity because of its high phenolic content (1388 mg EGA.100g-1 DW)while prickly pear peel powder (PPPP) showed relatively low content in both antioxidant activity and the total phenolics (421 mg EGA.100g- 1). The decrease in absorbance of β-carotene in the presence of different methanolic tested food by-products extracts (and wellknown antioxidants used as standards) with the oxidation of β- carotene and linoleic acid, antioxidant stability, indicated that PPP recorded the lowest decreasing followed by PPPP. The values of PPP absorbance through 120 min are coming well i.e. corresponding to the line of 50 mg.L-1 of α-tocopherol and upper to the line 50 mg.L-1 of BHT. By the same manner, PPPP is coming close to the line of 50 mg.L-1 of BHT. These data proved the high stability of the all tested food by-products when comparing with that more common standards, BHT and α- tocopherol. I.2 Relationship between bioactive compounds and antioxidant activity In the correlation analysis, important differences were found between phenolics, carotenoids and antioxidant activity of food by-products. When all food by-products were included in the statistical analysis, there was a positive significant (p≤ 0.05) relationship between total phenolics in PPPP (r2 = 0.636), total phenolics in PPP (r2 = 0.834), total carotenoids in PPPP (r2 = 0.531), total carotenoids in PPP (r2 = 0.593) and antioxidant activity. These correlations confirm that phenolic compounds with carotenoids are mainly responsible for the antioxidant activity of the tested by-products. Also, these data indicates that many other bioactive compounds beside phenolics and carotenoids such vitamins, fibers, minerals etc. probably contribute in the antioxidant activity of the tested by-products. II. Analysis of selected food by-products II.1 Proximate chemical composition The proximate chemical composition of selected food byproducts showed that the moisture content was ranged 5.71- 6.04%, total protein was 2.42 -10.54 %, crude fat was 0.69 -0.98 %, crude fiber was 7.84 -11.56 %, ash content was 3.12 -4.83 % and total carbohydrate content was 66.09- 80.22%. With the exception of carbohydrates, the PPP was recorded the highest content of the all chemicals components and could be a good source of protein, fiber and minerals. II.2 Physical properties The water (WHC) and oil (OHC) holding capacity of the tested food by-products noticed that PPP recorded the higher WHC than PPPP being 8.01and 5.33 g H2O.g-1, respectively, indicating that the higher fiber content in PPP hold more water compared to the PPPP. The behavior was recorded for the OHC. In similar study, the WHC and OHC of ROSP recorded the highest WHC followed by cauliflower leaves powder (CLP), PPP and MPP being 9.13, 8.39, 8.24 and 6.03 g H2O.g-1, respectively. II.3 Total dietary fiber, carotenoids and phenolics contents Total dietary fiber, carotenoids and phenolics contents of food by-products showed that the total dietary fiber content was ranged 39.53 and 45.91 g.100g-1, total carotenoids was 217.11 and 135.76 mg.100g-1 and total phenolics was 421 and 1388 mg EGA.100 g-1 for PPPP and PPP, respectively The PPP was recorded the highest content of total dietary fiber and total phenolics while PPPP recorded the highest values of total carotenoids. III. Effect of tested food by-products on the rheological parameters III.1 Farinograph parameters The incorporating of tested food by-products in dough increased the water absorption from 57.11% for control to 58.81 and 61.99% for dough contained 5% PPPP and PPP, respectively. The dough development time and dough stability increased from 2.7 to 3.3 and 3.9 min with 5% incorporation of PPPP and PPP, respectively this may be due to high content of dietary fibers in all of these by-products and pectin which act as a food hydrocolloid PPPP. The increasing dough development time illustrates that the dough with the addition of tested byproducts a longer relaxation time (the dough is tougher). Dough stability in minutes is the most important index for dough strength. Addition of tested food by-products, PPPP and PPP, to flour samples showed markedly longer stability periods than the control samples (flour without addition of food by-products). This affect was significantly with the addition of 5% of tested food by-products for wheat flour. from the viewpoint of dough farinograph quality number (FQN), statistically significant difference was found between the control sample (flour without the addition of the tested food by-products) and the dough with additions of 5% of tested food by-products (P < 0.05). It is meaning that an improvement in the quality of the dough occurred after the addition of 5% tested food by-products, when the FQN value significantly increased in comparison with the control sample. III.2 Extensograph parameters Dough strength (Extensibility) determined by the area under the curve and is proportional to energy needed to bring about rupture. The incorporating of tested food by-products in dough increased the extensibility from 171 mm for control to 179 and 187 mm for dough contained 5% PPPP and PPP, respectively. IV. Analysis of crackers enriched with tested plant byproducts IV.1 Sensory evaluation The PPPP and PPP crackers were significantly different (p≤0.05) in colour. The PPP crackers were shown to be the least liked by the panelists. For colour, among the tested food byproducts, the colour of PPPP crackers was rated the highest (7.10) by the panelists. The desirable colour of crackers is mainly due to the Millard browning during baking. However, in PPPP crackers, the colour could be partially contributed by the carotenoid in PPPP flour which imparts a yellowish colour to the crackers. Crispiness is perceived when food is chewed between molars, and is usually expressed in terms of hardness and factorability. In this study, there was no significant difference (p≤0.05) in crispiness amongst the different samples with different composite flour cracker types. However, the PPP crackers were significantly different (p≤0.05) in overall acceptance. IV.2 Bioactive components and antioxidant activity of control and composite flour crackers The bioactive components and antioxidant activity of control and enriched crackers with the tested by-products. The total dietary fiber, carotenoids and phenolics content in crackers 114 increased from 5.89, 3.01 and 110.23 to 8.11, 14.34 and 143.28 with 5% incorporation of PPPP and 8.74 g.100g-1, 7.88 mg.g-1 and 192.79 mg EGA.g-1 with 5% incorporation of PPP, respectively. In parallel, the antioxidant activity (AA) in control crackers was 30.11% which increased to 38.14 (26.67 as % of change) and 42.07 % (39.72 as % of change) with the incorporation of PPPP and PPP by 5%, respectively. Tested byproducts enriched crackers showed strong activity probably due to their high bioactive compounds (carotenoids and phenolics) content. |